玉竹提取物A对小鼠巨噬细胞白细胞介素-1和肿瘤坏死因子产生的影响

Effect of EA-PAOA on both IL-1 and TNF-α production of mice macrophages

目的研究玉竹提取物A(extraction A of polygonatum odoratum,EA-PAOA)对小鼠巨噬细胞和细胞因子产生的影响,以探讨对免疫功能的作用。方法检测小鼠体外巨噬细胞产生的白细胞介素-1(IL-1)和肿瘤坏死因子(TNF-α)。结果 10μg/mL以上浓度的EA-PAOA对小鼠巨噬细胞产生的IL-1有抑制作用,1000μg/mL浓度的EA-PAOA的抑制百分率(IP)达39.1%,对TNF-α抑制作用比对IL-1的抑制作用明显,10μg/mL浓度的EA-PAOA达28.6%,1000μg/mL浓度的EA-PAOA的CIP达78.6%。两者均呈量效依赖关系。结论 EA-PAOA对小鼠巨噬细胞产生的IL-1和TNF-α,均有抑制作用,可抑制小鼠的免疫功能。

Objective To research the effect of extraction a of polygonatum odoratum（EA-PAOA）on the cytokine production of mice macropphages（M）so as to explore the action of EA-PAOA on immunologic function.Methods To detect IL-1 and TNF-α produceted by mice M in vitro.Results EA-PAOA in the concentration of ≥ 10 μg /mL had the inhibitory action on IL-1 production of mice M.The inhibitory percentage（IP）in concentration of 1 000 μg /mL of EA-PAOA reached 39.1%.The inhibitory action of EA-PAOA on TNF-αproduction of mice M was higher than that on IL-1 production,the cytolytic inhibitory percentage（CIP）of EA-PAOA in concentration of 10 μg/mL reached 28.6%,and in concentration of 1000 μg /mL which reached 78.6%.The inhibitory action of EA-PAOA on both IL-1 and TNF-α of mice M depended on the concentration of EA-PAOA.Conclusion There is a inhibitory action of EA-PAOA on both IL-1 and TNF-α production of mice M.EA-PAOA inhibits the immunologic function of mice.