E-钙粘蛋白和MMP-9在胃癌组织中的表达及其临床意义

Expression and significance of E-Cadherin and matrix metalloproteinase-9 in gastric carcinoma

目的研究E-钙粘蛋白(E-Cadherin,E-Cad)和基质金属蛋白酶9(Matrix metalloprotein-ase-9,MMP-9)在胃癌中的表达,及其与胃癌临床病理特征、淋巴结转移和患者预后之间的关系。方法应用免疫组织化学方法,检测正常胃粘膜和胃癌标本的E-Cad和MMP-9表达。结果在胃癌组织中,E-Cad呈低表达,阳性表达率为53.7%,明显低于正常胃粘膜组织(P<0.05),其表达率与肿瘤大小、肿瘤分化、TNM分期显著相关(P<0.05),但与淋巴结转移无明显相关性(P>0.05);E-Cad表达阳性者,其5年生存期率较高(P<0.05)。MMP-9在胃癌组织中呈高表达(75.0%),明显高于正常组织,且与淋巴结转移、肿瘤大小、肿瘤分化、分期以及生存期显著相关(P<0.05)。结论胃癌广泛存在E-钙粘蛋白及MMP-9表达异常,且与预后密切相关。

Objective To investigate the expression of E-Cadherin（E-Cad） and matrix metalloproteinase-9（MMP-9） in gastric cancer and their relationship with clinical pathological parameters,lymph metastasis and prognosis.Methods The expressions of E-Cad and MMP-9 proteins were detected by immunohistochemistry.Results E-Cad expression was low in gastric cancer tissue compared with normal gastric mucosa.The positive expression rate was 53.7% which was significantly lower than normal gastric mucosa（P〈0.05）.Its expression correlated with tumor size,tumor differentiation,and TNM stage（P〈0.05）,but no significant correlation with lymph node metastasis was found（P〈0.05）.The 5-year survival in patients with positive E-Cad expression was significantly longer than those with negative expression（P〈0.05）.MMP-9 in gastric cancer tissues showed significantly higher expression（75.0%） than in normal tissue（P〈0.05）.Its expression was related to lymph node metastasis,tumor size,tumor differentiation,TNM stage and survival（P〈0.05）.Conclusion The expression of E-Cad and MMP-9 is related to prognosis of gastric carcinoma.