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猪圆环病毒2型SD/2008株的分离鉴定及培养特性 被引量：3

Isolation and identification of porcine circovirus type 2 and its cultivation characteristics
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摘要 应用PK15细胞从山东省某猪场分离到1株病毒,通过免疫过氧化物酶单层试验和全基因组序列分析,证明该分离株为猪圆环病毒2型(PCV-2,命名为SD/2008株),基因组全长1767bp,为PCV-2b基因型;感染PK15细胞后96h病毒含量最高,培养液含病毒基因组1.72×108copies/μL,连续培养120h不出现细胞病变;连传5代,病毒滴度稳定,维持在106TCID50/0.1mL左右;传代PK15细胞时同步接种病毒后18h,用300mmol/L的D-氨基葡萄糖处理细胞,病毒滴度可以提高5.62倍。 A strain of porcine circovirus type 2,named PCV-2 SD/2008 strain,was isolated with PK15 cell from a pig farm in Shandong province and identified by immunoperoxidase monolayer assay（IPMA） and complete genomic sequence analysis.Its genome contained 1 767 bp and belonged PCV-2b genotype;after 96 h innoculation,the content of virus in PK15 cell reached the highest,1.72×108 viral DNA copies per microlitre;and no cytopathogenic effect appeared within 120 h cultivation;the virus titer stayed at the level of about 106 tissue culture infective dose 50 （TCID50/0.1 mL） within passage 2 to 5 and increased by 5.62 times when the PK15 cells were treated with 300 mmol/L of D-glucosamine at the 18th hour after synchronized inoculation.
作者 刘祥义 王秀平 陈春花 宋勤叶 焦建达 冯秀 蒋丽釵 王昆
机构地区 河北农业大学动物科技学院 河北省邯郸市动物疫病预防控制中心
出处 《中国兽医学报》 CAS CSCD 北大核心 2010年第7期883-887,共5页 Chinese Journal of Veterinary Science
基金 河北省自然科学基金资助项目(C2008000285) 河北农业大学大学生科技创新活动基金资助项目
关键词 猪圆环病毒2型 分离鉴定 基因型 培养特性 porcine circovirus type2 isolation and identification genotype cultivation characteristics
分类号 S852.65 [农业科学—基础兽医学]
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参考文献19

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二级参考文献13

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共引文献146

同被引文献30

  • 1刘长明,张超范,危艳武,谭斌,陆月华,谷守林.猪圆环病毒2型细胞培养适应毒株的培育和鉴定[J].中国预防兽医学报,2006,28(3):248-252. 被引量：27
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引证文献3

二级引证文献8

中国兽医学报
2010年 第7期

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