摘要
在柔红霉素生物合成途径中,dnrU基因编码酮基还原酶,催化副产物(13S)-13-二氢柔红霉素的产生。本研究构建了dnrU基因的同框敲除质粒pYG1116,将其转入柔红霉素高产菌SIPI-HJ1001,通过同源重组双交换的方法将dnrU基因内部编码153个氨基酸的序列敲除,从而得到dnrU基因失活的突变株mYG1116。该突变株柔红霉素发酵单位比出发菌株约提高了52%。
The ketoreductase encoded by gene dnrU in daunorubicin biosynthesis pathway can transform daunorubicin to（13S）-13-dihydrodaunorubicin,which is a by-product for daunorubicin biosynthesis.An in-frame knockout plasmid was constructed and then transformed into a daunorubicin-producing strain SIPI-HJ1001 to inactivate dnrU by homologous recombination within dnrU.The production of daunorubicin by the recombinant strain mYG1116 was increased by 52% compared with the parental strain.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2010年第10期732-736,共5页
Chinese Journal of Pharmaceuticals
基金
国家"重大新药创制"科技重大专项资助(2009ZX09301-007)
