大豆异黄酮的抗氧化和抗肿瘤活性研究

Research on Antioxygenic and Antitumor Activities of Soybean Isoflavones

采用邻苯三酚自氧化法和Fenton法,检测大豆异黄酮的抗超氧自由基和羟基自由基的活性,通过MTT比色法检测大豆异黄酮对大鼠肝癌CBRH-7919细胞和小鼠白血病CML-K562细胞增殖的影响来研究大豆异黄酮的抗氧化和抗肿瘤活性。结果表明:大豆异黄酮具有显著的抗氧化和抗肿瘤活性。其中大豆异黄酮对超氧自由基的清除率达到50%时所需要的用量(IC50)为0.14 mg.mL-1;对羟基自由基的清除率达到50%时所需要的用量(IC50)为0.57 mg.mL-1;大豆异黄酮对CBRH-7919细胞生长抑制率达到50%时所需要的用量(IC50)为7.55 mg.L-1;对CML-K562细胞生长抑制率达到50%时所需要的用量(IC50)为10.35 mg.L-1。大豆异黄酮具有显著的抗氧化和抗肿瘤活性。

To investigate the bioligical activities of soybean isoflavones, pyrogallol autoxidation method and Fenton method were used to detect whether soybean isoflavones could scaverge hydroxyl free radical and superoxide free radical. MTT assay was used to examine the effect of soybean isoflavones on cell viabilities. The results sbowed that soybean isoflavones exbibited antioxygenic activities,the antitumor and antibacterial activities 50% elimination concentration of soybean isoflavones on su- peroxide free radical and hydroxyl free radical was 0.14 mg · mL-1 and 0. 57 mg · mL-1. Soybean isoflavones could inhibit CBRH-7919 cell and CML- K562 cell growth in a dose-depent manner, 50% inhibitory concentration（IC50） on CBRH-7919 cell was 7.55 mg · L-1 ,and ICs0 on CML- K562 cell was 10.35 mg · L-1. Soybean isoflavones exhibited antioxygenic,antitumor and antibacterial activities.