荧光光谱法探索多巴酚丁胺与BSA的相互作用

Interaction Between Dobutrex and Bovine Serum Album by Fluorescent Spectra

用荧光光谱法研究在人体生理pH条件下药物多巴酚丁胺与牛血清白蛋白(BSA)的相互作用。结果表明,多巴酚丁胺对BSA内源性荧光有较强的猝灭作用,且猝灭类型为静态猝灭。根据猝灭结果求得了298、301、304 K3个不同温度下多巴酚丁胺与牛血清白蛋白结合作用的结合位点数、结合常数以及反应的热力学参数,由此确定出2者之间的主要作用力为疏水作用力。而应用竞争实验确定了多巴酚丁胺在BSA上的结合位点为II位;用同步荧光光谱法探讨多巴酚丁胺对BSA构象的影响。

The interaction between dobutrex and bovine serum albumin（BSA） was studied by fluorescence spectra under the simulated physiological condition.It was shown that this compound has a quite strong ability to quench the intrinsic fluorescence of BSA.The quenching mechanism was static quenching procedure.The fluorescence quenching data were analyzed according to Stern – Volmer equation and Line weaver-Burk equation,thermodynamic parameters,binding constants Ka,the number of binding sites n were evaluated at 298,301 and 304 K,respectively.The main sorts of acting force between the drug and BSA was found to be hydrophobic force.The site markers competitive experiments indicated that the binding of dobutrex and bovine serum albumin primarily in site II.The effect of dobutrex on the conformation of BSA was analyzed by synchronous fluorescence.