氧化海藻酸钠交联生物性组织制备生物材料的实验研究

Experimental study of fixing biological tissues for biomaterials by oxidized sodium alginate

探讨经氧化海藻酸钠(ADA)处理的猪主动脉的性能特点,为构建人工器官等提供理想的生物材料。用ADA处理猪主动脉4～72h,交联制得的生物材料测定交联指数并进行形态学观察,同时检测其力学性能。结果显示ADA既有效地去除和降低了组织中产生抗原性的细胞成分和自由氨基,又保持了天然组织构架的完整;经它处理的血管组织具有较好的韧性、黏弹性,是一种良好的生物材料;其中以在pH为4.0,ADA浓度为15g/100mL的条件下,交联72h得到的材料显示出更好的性能。

To investigate the characteristics of porcine aorta fixed with oxidized sodium alginate（ADA）to provide the ideal biomaterials for constructing artificial organs.The porcine aortas were treated by ADA for 4hto 72h.The biological materials fixed with ADA were then examined with light microscope and SEM.Subsequently,the fixation index determination and biomechanics test were also performed.The experimental results indicated that the antigenicity of porcine aorta tissues could be diminished by ADA through getting rid of cell in the porcine aorta tissues or reducing the level of free amino groups in the porcine aorta tissues.The structural integrity of porcine aorta tissues can be preserved after treating with ADA.It was also found that the ADA-fixed porcine aorta tissues appeared more toughness and visco-elasticity,and should be promising biomaterials.Among the porcine aorta tissues fixed with ADA,the tissues that were fixed at pH 4.0,15g/100mL initial fixative concentrations for 72hshowed a more favorable characteristic.