内皮细胞在钛金属表面透明质酸微图形上的粘附行为

Behaviour of HUVECs adhered on patterned HA on titanium

采用软刻蚀的方法在钛表面制备透明质酸(Hyaluronic acid,HA)微图形,利用高分子量的HA可以阻抗细胞粘附的性质,将内皮细胞粘附限制在一定的区域,并研究了内皮细胞的形态和细胞骨架排列情况。结果表明,HA可以通过软刻蚀的方法在钛金属表面得到轮廓清晰的的微米级小尺寸图形,该尺寸的微图形可以有效地将细胞限制在一定的区域,并能引导细胞朝一定的方向伸长,细胞骨架排列分布与细胞形态一致。这种改变细胞形态的简易方法为进一步的细胞功能及内皮化的研究提供了新的手段。

Micropatterned hyaluronic acid（HA）was fabricated by the method of soft-lithography on the surface of titanium.Endothelial cells（ECs）were confined to a certain place due to the HA of high molecular weight with the resistance property of cell adhesion.Then,the the morphology and cytoskeletal conformation of ECs were investigated.The results indicated that micropatterned HA with microscale and with good edge definition were successfully formed on titanium substrates.ECs were confined to a certain place not covered with HA effectively and elongated,the cell＇s actin was consistent with its morphology.The simple method made cells’morphology changed could be used for both further research of cells＇function and endothelialization.