CD30在强直性脊柱炎患者外周血T细胞亚群的表达及意义

Determination of CD30 on peripheral blood T cell subsets in ankylosing spondylitis patients

目的研究CD30在强直性脊柱炎(AS)患者外周血T细胞亚群的表达及意义,分析AS患者免疫学发病机制。方法健康对照组24例,患者组CT检查AS患者骶髂关节并分为Ⅰ(11例)、Ⅱ(12例)和Ⅲ级(10例)。采用ECD标记抗CD3单抗,PE-Cy5标记抗CD4、CD8单抗以及FITC标记抗CD30单抗作三色流式细胞术;CD3/CD4、CD3/CD8设门,分析CD30在AS患者外周血CD4+T、CD8+T细胞的表达水平。结果健康对照比较,CD4+CD30-T细胞在Ⅰ、Ⅱ级AS患者中均明显降低(P<0.05),在Ⅲ级AS患者中则明显升高(P<0.05);CD8+CD30-T细胞在Ⅱ级AS患者明显升高(P<0.05);CD8+CD30+T细胞在AS各级患者中均明显升高(P<0.05)。结论 AS各级患者外周血T细胞亚群比例失衡与患者外周免疫功能紊乱及疾病进程有关。

Objective To determine the CD30 expression on peripheral blood T lymphocyte subsets in patients with ankylosing spondylitis（AS）.Methods The ankylosing spondylitis patients were diagnosed in accordance with 1984′ diagnostic criteria of ankylosing spondylitis and divided into gradeⅠ（11 cases）,Ⅱ（12 cases） and Ⅲ（10 cases） as computerized tomography（CT）.The percentages of CD4＋CD30-T,CD4＋CD30＋T,CD8＋CD30-T,CD8＋CD30＋T in peripheral blood of AS patients and healthy control（24 cases） were analyzed by immunofluorescence staining and three-color flow cytometry（FCM） using CD3/CD4,CD3/CD8 gating in vitro.Results The percentages of CD4＋CD30-T in AS gradeⅠand Ⅱ were clearly lower than healthy control（P0.05） while those in AS gradeⅢ were obviously higher than healthy control（P〈0.05）.Meanwhile the percentages of CD4＋T,CD8＋T and the ratios of CD4＋T/ CD8＋T in AS grade Ⅲ changed little as compared with healthy control（P〉0.05）.The percentages of CD8^＋CD30^-T in grade Ⅱand the percentages of CD8^＋CD30^＋T in all of AS patients were more higher than healthy control（P〈0.05）.Conclusion It is suggested that the cellular immunity and humoral immunity may be disorder in AS patients.The loss of balance between T lymphocyte subsets may play an important role in the immunopathogenesis of AS and be correlated to the proceeding of patient′s condition closely.