摘要
试验首先建立了30日龄猪皮下脂肪和背最长肌组织块体外培养体系,共设2个处理,处理1为阴性对照(添加0.1mmol/l牛血清白蛋白,BSA),处理2添加100μmol/l的t10,c12-CLA,每个处理设6个平行,接种时即加入BSA与t10,c12-CLA进行处理至第10d,试验结束后收集细胞保存备用。采用荧光定量PCR技术及试剂盒检测研究t10,c12-CLA对猪皮下和背最长肌脂肪代谢关键酶(FAS、ME、LPL、HSL)和激素(INSR、GHR)及甘油三酯(TG)合成的影响。结果表明:(1)100μmol/lt10,c12-CLA显著降低了猪皮下脂肪TG含量并提高了猪背最长肌的TG含量(P<0.05);(2)100μmol/lt10,c12-CLA显著抑制了猪皮下脂肪FAS、INSR与背最长肌HSL和LPL的基因表达,促进了猪皮下脂肪HSL和背最长肌INSR的基因表达(P<0.05);(3)本研究结果从脂肪代谢关键酶类与调控激素方面揭示了t10,c12-CLA对猪皮下和背最长肌组织脂肪代谢与沉积的差异性调控机制,进一步证实了t10,c12-CLA抑制猪皮下脂肪沉积同时提高肌内脂肪含量。
In vitro tissue culture system of subcutaneous（SC） adipose and longissimus muscle taken from 30-days pig were constructed firstly,0.1 mmol/l bovine serum albumin （BSA,as control） ,100μmol/L t10,c12-CLA were added to the cultures for 10 d,six replicates were performed for each treatment. At the end of a 10-d treatment period,cells were harvested and stored at-70℃. Real-time RT-PCR and kit assay were used to characterize the effects of t10,c12-CLA on the lipogenic and lipolytic enzymes ,regulation hormones,TG content in longissimus muscle and SC adipose of pig. The results showed that：the mRNA abundance of FAS and INSR,TG content in SC adipose tissue and the mRNA abundance of HSL and LPL in longissimus muscle tissue were decreased by adding t10,c12-CLA （P0.05）;by contrast,HSL mRNA abundance in SC adipose tissue and the mRNA abundance of INSR,TG content in longissimus muscle tissue were increased by adding t10,c12-CLA（P0.05）. These results illustrated that the effects of t10,c12-CLA on lipid metabolism and deposition of SC adipose tissue differed from that on longissimus muscle tissue ,and confirmed t10,c12-CLA inhibited the lipid deposition of SC adipose and promoted the lipid deposition of longissimus muscle in pig further.
出处
《饲料工业》
北大核心
2010年第19期12-16,共5页
Feed Industry
基金
国家"973"重点基础研究发展计划(2004CB117500)
