噻唑橙核酸荧光探针的合成及光谱性质研究

Synthesis and spectral properties of thiazole orange compounds as DNA fluorescent probes

本文合成了一类可用于核酸分子检测的噻唑橙类(thiazole orange,TO)菁染料4a、4b和5,并对染料结构进行了表征。其中染料4a未见文献报道。三种染料在Tris-HCl(pH=7.0)缓冲溶液中的最大吸收光谱值分别在504nm、502nm、507nm处。荧光发射光谱表明:染料在Tris-HCl(pH=7.0)缓冲溶液中无荧光,加入ds-DNA后荧光显著增强,荧光增强与加入ds-DNA的量之间呈现良好的线性关系。改变染料取代基后得到的染料4a对ds-DNA表现出更优异的荧光增强性能。

The thiazole orange fluorescent dyes 4a,4b and 5 were synthesized and characterized for analysis of nucleic acid.Dye 4a was novel,and no previous paper had reported about it.Maximum of absorption spectra of the three dyes in Tris-HCl（pH = 7.0） was at about 504nm、502nm、507nm respectively.Fluorescent spectra of dyes together with ds-DNA showed that the dyes showed no fluorescence in Tris-HCl（pH = 7.0）.Addition of the ds-DNA enhanced the fluorescence of the dyes with a good linear correlation between the fluorescent intensity of dye and the volume of ds-DNA.The modifying of the substitutes for dye 4a showed that dye 4a performed better fluorescent intensity.