16S rDNA序列分析鉴定一株芽孢杆菌

Identification of One Strain of Bacillus Using 16S Ribosomal DNA Sequence

利用16S rDNA分析对芽孢杆菌Y-2进行系统进化鉴定。首先提取菌株Y-2的基因组DNA,经PCR扩增后,测定其16S rDNA序列,将测序结果与NCBI上已知菌种的16S rDNA序列进行BLAST对比,构建系统进化树进行分析,最终鉴定该菌株为枯草芽孢杆茵(Bacillus subtilis),其同源性≥99%。

Using the 16S rDNA sequence analysis to identify a bacillus which was named Y-2,Pab02.Firstly the genomic DNA from Y-2 was extracted,and then the target 16S rDNA sequence was amplified and the amplified sequence was analyzed by the NCBI with BLAST data base.At last,according to its position in phylogenetic tree,Y-2 was finally identified as Bacillus subtilis.