吴茱萸次碱对硝基氯苯诱导的Nc／Nga小鼠特应性皮炎样皮损的影响

Effect of Rutaecarpine on 2, 4 - dinitrochlorobenzene - induced Atopic Dermatitis - like Skin Lesions in NC

目的观察吴茱萸次碱对特应性皮炎动物模型Nc／Nga小鼠的作用。方法外用2，4-二硝基氯苯（DNcB）反复刺激Nc／Nga小鼠建立特应性皮炎动物模型，通过观察皮肤组织病理学切片、免疫学指标评价皮炎损伤程度，观察吴茱萸次碱对特应性皮炎动物模型Nc／Nga小鼠的治疗作用。结果外用DNCB可以引起Nc／Nga小鼠湿疹样皮炎的产生；模型组小鼠血浆IgE水平（124．42±11．14ng／m1）较正常对照组（17．22±3．56ng／m1）增高（P〈0．05）；治疗组IL-12和IFN-γ水平（62．12±3．14pg／ml，68．29±1．39pg／m1）较模型组血浆IL-12和IFN-γ水平（20．14±6．15pg／ml，51．23±1．45pg／m1）增高，IgE水平（69．17±4．15ng／m1）较模型组IgE水平下降（P〈0．05）；醋酸地塞米松组血浆IL-12、IFN-γ和IgE水平（59．15±1．24pg／ml，64．12±1．19pg／ml，6817±1．15ng／ml）与治疗组比较，差异无统计学意义（P〉0．05）。结论吴茱萸次碱可能通过促进IL-12和IFN-γ分泌，抑制IgE的合成，发挥治疗NdNga小鼠特应性皮炎样皮损的作用。

Objective To study the effect of Rutaecarpine on the treatment of atopic dermatitis in Nc/Nga mouse modeIs. Methods 2, 4 - dinitrochlorobenzene （DNCB） was repeatedly applied on the back of NC/Nga mice to establish the animal models of atopic dermatitis. Atopic dermatitis- like skin lesions and the effect of Rutaecarpine on the atopic dermatitis in NC/Nga mouse models were evaluated by skin histopathology and immunological parameters. Results Topical DNCB may induce eczematous dermatitis in Nc/Nga mice. The plasma level of IgE in model group was significantly higher than that in control group （124.42 ± 11.14 ng/ml vs. 17.22 ± 3.56 ng/ml, P 〈 0.0.5）. The plasma levels of IL - 12 and IFN -γ （62.12 ±3.14 pg/ml, 58.29 ± 1.39 pg/ml） in treatment group were significantly higher than those in model group （20.14 ±6. ]5 pg/ml,51.23 ± 1.45 pg/ml）. The plasma level of IgE （69.17± 4.15 ng/ml） in treatment group was lower than that in model group （P 〈 0.05）. There was no statistically significant difference in the plasma levels of IL- 12, IFN- γ and IgE between treatment group and positive medicament group （P 〉 0.05）. Conclusions Rutaecarpine might inhibit the IgE synthesis and play a therapeutic effect on atopic dermatitis-like skin lesions in NO/Nga mice through promoting the secretion of IL - 12 and IFN - γ.