戊唑醇原药诱变性与亚慢性经口毒性的实验研究

Experimental Study on Mutagenicity and Subchronic Toxicity by Oral Exposure of Tebuconazole TC

目的探讨戊唑醇原药的诱变性及亚慢性毒性作用。方法按照我国GB15670-1995《农药登记毒理学试验方法》要求进行。结果 62.5-250μg/皿的Ames实验结果为阴性;染毒14.7-294 mg/kg的剂量组微核试验结果为阴性;染毒36.8-147 mg/kg的剂量组的小鼠睾丸精母细胞染色体畸变率未见增加;亚慢性经口毒性试验高剂量组（71.7mg/kg.bw/d）的雄鼠在染毒中后期出现精神不振、少动、被毛蓬松、会阴部污秽等中毒症状。结论戊唑醇原药无明显致突变作用。戊唑醇原药有一定的蓄积毒性作用,可引起潜在的慢性中毒,主要的毒作用部位是肝脏与血液系统。戊唑醇原药SD雌、雄大鼠亚慢性（90 d）经口试验未观察到有害效应的剂量水平分别为16.3、8.0 mg/kg.bw/d。

Objective To explore the mutagenicity and subchronic oral toxicity of Tebuconazole TC.Methods The experiment was conducted according to the rules from ＂Toxicological Test Methods of Pesticides for Registration＂（GB15670-1995 of PRC） Results 62.5～250 μg/plate of emamectin benzoate TC did not induce positive mutations of strains TA97,TA98,TA100,TA102 in Ames test.Mice bone marrow cell micronucleus test（dosage： 14.7～294 mg/kg） showed that each dosage did not show significant difference in the rate of marrow cell micronuclei in comparison with the negative control group and so did the mice testicle cell chromosome aberration test（dosage： 36.8～147 mg/kg）.The results from subchronic toxicity test showed that male rats treated with high dosage（71.7 mg/kg.bw/d） appeared obvious clinical symptoms such as poor spirit,blowzy fur,and filth in perineal region which beginning from the seventh weeks until to the exposure end.Conclusions Tebuconazole TC does not induce significantly mutagenic effect.Tebuconazole TC may have some cumulative toxicity and could provoke potential chronic toxicity,the main target organs are liver and hematological system.The no-observable-adverse-effect levels（NOVEL） of Tebuconazole TC in subchronic toxicity test by oral exposure were 8.0 mg/kg/d and 16.3 mg/kg/d,respectively.