摘要
目的:探讨手术前后卵巢癌患者外周血T细胞亚群及CD4+CD25+调节性T细胞的变化及其临床意义。方法:采用流式细胞术检测80例卵巢癌患者外周血T淋巴细胞亚群、NK细胞及CD4+CD25+调节性T细胞水平。结果:与对照组相比,卵巢癌患者外周血中CD3+,CD4+,CD4+/CD8+比值及NK细胞含量均降低(P<0.05),CD8+升高(P<0.05);随着疾病进展Ⅲ期+Ⅳ期与Ⅰ期+Ⅱ期比较CD3+,CD4+,CD4+/CD8+比值及NK细胞含量均降低(P<0.05),CD8+升高(P<0.05)。卵巢癌患者外周血CD4+CD25+细胞比例高于正常对照组(P<0.05);手术后卵巢癌患者T细胞亚群比例与术前比较并未明显恢复(P>0.05),但CD4+CD25+调节性T细胞水平有所恢复(P<0.05)。结论:卵巢癌患者免疫功能低下,术后短期并不能恢复,CD4+CD25+调节性T细胞水平升高,术后明显恢复,提示其在免疫耐受中起重要作用。
Objective：To explore the changes of T lymphocyte subsets and CD4＋CD25＋ regulatory T cells in peripheral blood and their clinical significances in patients with ovarian carcinoma before and after operation.Methods：The levels of T lymphocyte subsets,NK cells and CD4＋CD25＋ regulatory T cells in peripheral blood of 80 patients with ovarian carcinoma were detected by flow cytometry.Results：Compared with control group,the levels of CD3＋ regulatory T cells,CD4＋ regulatory T cells,the ratio of CD4＋/CD8＋ and the content of NK cell decreased（P0.05）,the level of CD8＋ regulatory T cells increased（P0.05）;compared with stage I and stage Ⅱ,the levels of CD3＋ regulatory T cells,CD4＋ regulatory T cells,the ratio of CD4＋/CD8＋ and the content of NK cell at stage Ⅲ and stage IV decreased with the progress of disease（P0.05）,the level of CD8＋ regulatory T cells increased（P0.05）;the level of CD4＋CD25＋ regulatory T cells in peripheral blood in ovarian carcinoma group was significantly higher than that in control group（P0.05）.There was no significant difference in levels of T lymphocyte subsets before and after operation（P0.05）,but the level of CD4＋CD25＋ regulatory T cells recovered after operation（P0.05）.Conclusion：The immune function of patients with ovarian carcinoma is low,and they can＇t recover in short time after operation,the increase of CD4＋CD25＋ regulatory T cell level and obvious recovery after operation indicate that it plays an important role in immune tolerance.
出处
《中国妇幼保健》
CAS
北大核心
2010年第30期4363-4364,共2页
Maternal and Child Health Care of China
基金
吉林省科学技术厅立项项目〔200705445〕
