siRNA干扰半乳凝素-3后对食管癌Eca-109细胞株增殖的影响

Effects on cell growth of Eca-109 esophageal carcinoma cell line after transfection of Galectin-3-siRNA

目的探讨siRNA干扰半乳凝素-3(Galectin-3)mRNA后,食管癌Eca-109细胞株增殖活性的影响。方法将Galectin-3-siRNA转染食管癌Eca-109细胞株后(转染组成2、3),分别用流式细胞术、免疫细胞化学和MTT检测转染效率、Galectin-3蛋白IOD值、OD值和细胞增殖抑制率。结果转染组食管癌Eca-109细胞株Galectin-3蛋白IOD值、OD值和细胞增殖抑制率与空白对照组和阴性对照组比较,差异有统计学意义(P<0.01)。结论运用siRNA可以有效抑制Galectin-3蛋白的表达及细胞增殖活性,Galectin-3参与食管癌Eca-109细胞株的增殖,Galectin-3可成为治疗食管癌的靶基因。

Objective To observe the proliferation activity of Eca-109 cell line after transfected by Galectin-3 siRNA. Methods The Galectin-3 siRNA chain was synthesized and transfected into Eea-109 cell line by Lipofectamine2000, flow cytometry was used to detect the transfection efficiency of siRNA, and immuocytochemistry was used to analyze the quantity of Galectin-3 protein, and MTT was used to detect the proliferation activity of Eca-109 cell line. Results The obvious down-regulation of the Galectin-3 protein was found following transfection of Galectin-3 siRNA chain, and the proliferation activity of Eca-109 cell line was inhibited. Conclusion The transfection of synthesized Galectin-3 siRNA in Eca-109 cell line can down-regulate the expression of Galectin-3 protein and the proliferation activity of Eca-109 cell line, which indicates that Galectin-3 is involved in esophageal cancer Eca-109 cell line proliferation, and Galectin-3 gene can be used as a new target gene in treating the esophageal cancer.