肿瘤坏死因子-α对大鼠肺成纤维细胞表达结缔组织生长因子的影响

The influence of tumor necrosis factor-α (TNF-α) on expression of rat lung fibroblasts of CTGF

目的观察肿瘤坏死因子-α(TNF-α)对大鼠肺成纤维细胞表达结缔组织生长因子(CTGF)的影响。方法一次性气管内滴注博莱霉素-A5制作肺纤维化模型,采用组织块法体外培养肺成纤维细胞;分为4组:对照组、TNF-α5,10,20μg/L组,采用免疫细胞化学技术和流式细胞术检测不同浓度TNF-α对大鼠肺成纤维细胞表达CT-GF的影响。结果与对照组比较,不同浓度的TNF-α均能够促进大鼠肺成纤维细胞表达CTGF(P<0.05),20μg/LTNF-α的促表达能力最强。结论 TNF-α可促进体外培养的大鼠肺成纤维细胞表达CTGF。

Purpose To investigate the influence of tumor necrosis factor-α（TNF-α）on the expression of rat lung fibroblasts of CTGF.Methods By drop Bleomycin A5 hydrochloride in the intratracheal pulmonary fibrosis model was made and the issues explanted to culture lung fibroblasts were adopted.It is divided into four groups including control group,5 μg/L TNF-α,10 μg/L TNF-α and 20 μg/L TNF-α.The research will adopt the method of immunocytochemistry and flow cytometry to detect the influence of different density of TNF-α on the rat lung fibroblasts of CTGF.Results Compared with the control group,different densities of TNF-α are all able to promote expression of rat lung fibroblasts of CTGF（P0.05）.20 μg/L TNF-α has the strongest ability in promoting the expression of rat lung fibroblasts of CEGF.Conclusion TNF-α could promote the in vitro cultured expression of rat lung fibroblasts of CEGF.