异种异体脱细胞神经支架制备的实验研究

Experimental Study on Preparation of Xeanogenic Allogenic Acellular Nerve Scaffold

目的:探讨用化学方法制备异种异体脱细胞神经支架,为周围神经组织工程提供有效的神经支架。方法:A组,SD大鼠5只,体重200～250g,取双侧坐骨神经;B组,家兔1只,体重500～600g,取周围神经10根,与A组等粗等长;C组,家犬1只,取周围神经10根,与A、B组等长等粗。三组神经均采用TritonX-100、脱氧胆酸钠等进行脱细胞处理。用HE染色对去细胞程度、脱髓鞘程度及神经管道完整性进行评价。结果:HE染色A、B、C组轴突及细胞核均消失,神经内膜形成不规则圆形空腔。结论:在TritonX-100、脱氧胆酸钠的处理下,A、B、C组的细胞均被脱净,可以作为组织工程神经的支架。

Objective：To explore the preparation of xenogeneic allogenic acellular nerve stand by a chemical method to provide an effective tissue-engineered nerve scaffold.Methods：The bilateral sciatic nerves were taken from 5 of the rats,weighing 200-250g,as group A.The 10 rabbit peripheral nerves were taken from a rabbit,weighing 500-600g,as group B.10 of peripheral nerves were taken from a dog as group C.All the nerves＇ sizes are same.The nerves from group A,B and C were treated by Triton X-100 and sodium deoxycholate for removing the cells around the nerves.Then HE staining was used to evaluate the acellular degree,demyelination degree and nerve pipeline integrity.Results：The result shown that the axons and the nuclei disappeared in all the nerves of 3 groups.And the endoneurium formed an irregular circular cavity.Conclusion：After treated with Triton X-100 and sodium deoxycholate,the cells around the nerves from group A,B and C were de-net and the nerves can be used as an effective tissue-engineered nerve scaffold.