摘要
目的:考察闪式与回流提取对黄芪皂苷提取工艺的影响。方法:以黄芪甲苷提取率和出膏率作为考察指标,采用HPLC-ELSD法作为测定方法,以Welc C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈:水(40∶60)为流动相,流速1 mL.min-1,漂移管温度80℃,气流量50 psi,考察闪式与回流提取对黄芪皂苷提取工艺的影响。结果:闪式提取所得黄芪甲苷含量高于回流提取。结论:闪式提取黄芪皂苷不仅提取率高,而且耗时短,节省能源,可望广泛地应用在制药工业上。
Objective：To study smashing and reflux extraction of astragaloside.Method： The extraction process was investigated by smashing and reflux extraction with the extraction of astragalosideⅣ and the rate of extracts as criteria.The measurement conditions were used as follows： Welc C18（4.6 mm × 250 mm,5 μm） column was used with acetonitrile：water（40∶ 60） as mobile phase;the flow rate was 1 mL.min-1;the temperature of drift tube was set at 80 ℃;gas flow was 50 psi.Result： The conent of astragalosideⅣ of smashing extraction were higher than reflux extraction.Conclusion： Smashing extraction method for astragaloside can save time,working and energy,and it is hoped to be widely used in the pharmaceutical industry.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第13期1-3,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大新药创制专项(2009ZX09103-307)
关键词
闪式提取
黄芪
黄芪甲苷
提取工艺
smashing extraction
Radix Astragali
astragalosideIV
extraction process
