HPLC同时测定小蓟中蒙花苷和绿原酸的含量

Determination of Contents of Chlorogenic Acid and Acaciin in Cirsium setosum (willd.) MB. by HPLC Method

目的:建立同时测定小蓟中绿原酸和蒙花苷含量的测定方法,并用此方法测定不同产地小蓟药材的含量。方法:HPLC法,采用Diamonsil C18(4.6 mm×200 mm,5μm)为色谱柱;以甲醇为流动相A,以水(含1%冰乙酸)为流动相B,梯度洗脱;流速1.0 mL·min-1;柱温35℃;进样量20μL;检测波长为327 nm。结果:蒙花苷、绿原酸的浓度与峰面积成良好的线性关系,蒙花苷、绿原酸线性范围分别为0.018～0.18 g·L-1(r=1)和0.005～0.05 g·L-1(r=0.9996);加样回收率分别为99.6%和99.3%,RSD分别为0.5%和1.1%。结论:该法简便,快捷,结果准确、重复性好,可用于小蓟药材中蒙花苷和绿原酸的质量控制。

Objective：To develop a HPLC method for the determination of chlorogenic acid and acaciin from common cephalanoplos herb.Method：Samples were analyzed by Diamonsil C18（4.6 mm×200 mm,5 μm） with methanol-water（1% glacial acetic acid） as mobile phase in gradient elution,327 nm as detected wavelength,1.0 mL.min-1 as flow-rate,35 ℃ as temperature of column,20 μL as injected volume.Result：The linear range of chlorogenic acid and acaciin was 0.005-0.05 g·L^-1（r=0.999 6） and 0.018-0.18 g·L^-1（r=1）,and the average recovery was 99.3% with RSD 1.1% and 99.6% with RSD 0.5% respectively.Conclusion：The method was rapid,convenient,accurate,and suitable for the quality control of contents of chlorogenic acid and acaciin in Cirsium setosum（willd.） MB.