和厚朴酚在缓冲溶液HBSS中的溶解性和稳定性

Study on Solubility and Stability of Honokiol in Buffer Solution of HBSS

目的:考察和厚朴酚在HBSS溶液中的溶解性和稳定性,为和厚朴酚在体内的吸收与代谢研究提供依据。方法:采用RP-HPLC法,分别考察了pH、温度、光照对和厚朴酚溶解性和稳定性的影响。结果:和厚朴酚在甲醇的标准曲线为Y=4 764.5X-10 955(r=0.999),在4～200μmol·L-1与峰面积呈良好的线性关系,检测限为0.2μmol·L-1,精密度和稳定性符合要求。和厚朴酚在含有1%甲醇和0.1%的吐温-80的HBSS中的溶解性较好,和厚朴酚在酸性中的稳定性优于碱性环境,在pH 7.4的HBSS中的稳定性比较理想,光照对和厚朴酚的稳定性没有影响。结论:和厚朴酚在pH 7.4的HBSS中的溶解性和稳定性比较理想,当其达到溶解平衡之后于-20℃储存。

Objective：To investigate the solubility and stability of honokiol in HBSS,Establish the basis of the drug metabolism research.Method：The concentration of honokiol was determined by RP-HPLC method,pH,temperatures and light were investigated on the solubility and stability of honokiol.Result：The linear calibration curve of Honokiol in methanol in the concentration range of 4-200 μmol·L^-1 with a detection limit of 0.2 μmol /L,The equation was calculated to be Y = 4 764.5X-10 955（r=0.999）,The precision and stability accorded with the requirements of methodology.The solubility of honokiol is good in HBSS containing 1% methanol and 0.1% Tween-80,the stability of honokiol in HBSS of acidic is more better than HBSS of alkaline,.light on the stability of honokiol has no influence.Conclusion：Tthe solubility and stability of honokiol in HBSS of pH 7.4 is rather ideal,when it reached the dissolution equilibrium it should be storied at-20 ℃.