攻癌夺命组方对肝癌细胞系HepG2的杀伤作用研究

Observation on Cytotoxic Effect of Herb Decoction Gongai Duoming Tang to Hepatocellular Carcinoma Line HepG2

目的:探讨中药组方攻癌夺命汤对肝癌细胞系HepG2的杀伤作用及作用机制。方法:攻癌夺命汤煎剂与肝癌细胞系HepG2共培养后,分别用MTT法检测煎剂对肝癌细胞的杀伤作用,用RT-PCR方法检测缺氧诱导因子HIF1α(Hypoxia-inducible factor 1α)、凋亡相关分子Bax和Bcl-2等基因的mRNA表达变化。结果:攻癌夺命汤对肝癌细胞系HepG2的杀伤作用明显,且随浓度增高而增强,按生药量计6.375 g·L-1药液质量浓度3 d时的细胞毒作用达到80%以上;药物作用使HepG2的HIF1α的表达迅速升高,12 h即达到3倍以上;凋亡相关分子的变化在不同药液浓度有所差别:在2.125 g.L-1药液作用时Bax的增加幅度要大于Bcl-2。在4.250 g·L-1药液作用下,在早期Bcl-2和Bax都增加,24 h后二者表达均下降。结论:中药组方攻癌夺命汤对肝癌细胞系HepG2有很强的杀伤作用。药物作用后缺氧诱导因子HIF1α迅速升高提示其作用机理可能是影响到细胞呼吸所致。药物可能对细胞凋亡有一定作用。

Objective：To investigate the effect of the herb decoction Gongai Duoming Tang（GADMT） on hepatocellular carcinoma（HCC） cell line HepG2.Method：MTT was used to detect the cytotoxicity of the decoction to HepG2 and RT-PCR was used to detect the expression of hypoxia-inducible factor 1α（HIF1α） and the apoptosisrelated genes Bax and Bcl-2 in HCC cell line after treated by GADMT decoction.Result：MTT demonstrated that the GADMT decoction had a strong cytotoxicity to HepG2 as much as 80% after treating the cells with 6.375 g·L^-1 of concentration;the treatment also increased the expression of HIF1α promptly at 3 h and as high as 3 times at 24 h later;but the change of the Bax as well as Bcl-2 were more sophisticated.Conclusion：The GADMT decoction has a strong cytotoxic effect on HCC cell line HepG2 and the mechenism might be related with interruptting or damaging the cell respiration since a prompt increase of the expression of HIF1α.The decoction may have also an influence on the cell apoptosis.