黄芩及红花水提物对快速老化模型小鼠的抗衰老作用研究

Research on Anti-senile Action of Aqueous Extract of Scutellaria and Carthamus tinctorius in Senescence Accelerated Mouse

目的:采用快速老化模型小鼠P/8(SAM-P/8),探讨黄芩和红花水提物的抗衰老作用。方法:将SAM-P/8小鼠随机分为对照组(ig生理盐水),阳性药组(ig Vit E)黄芩和红花水提物高、中、低剂量组,均ig 4周。末次给药1 h后取胸腺和脑称重并计算脏器系数;测定小鼠血清及脑组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。结果:黄芩、红花水提物显著降低衰老小鼠的胸腺系数和脑系数,还可不同程度的提高衰老小鼠体内SOD,GSH-Px,CAT活性,抑制MDA含量的升高。结论:黄芩及红花水提物可能通过提高机体的抗氧化能力而达到抗衰老的作用。

Objective：To observe the anti-senile action of aqueous extract of Scutellaria and Carthamus tinctorius by using senescence accelerated mouse（SAM-P /8）.Method：SAM-P /8 were randomly divided into 8 groups： control group with saline only the positive treatment group（ig Vit E）,low,moderate,and high dose groups of aqueous extract of Scutellaria and C.tinctorius.The treatment groups were consecutively received by intragastric administration for 4 weeks.Thymus and brain were collected to weigh and calculate organ indexes 1 h after last administration.The activities of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,catalase（CAT） and the contents of malondi-aldehyde（MDA） in serum and brain tissue of all groups were determined by kit.Result：Aqueous extract of Scutellaria and C.tinctorius could elevate the brain and thymus indexes.They could also improve the activities of SOD,CAT,GSH-Px to different degree and decrease the contents of MDA.Moderate dosage of Scutellaria and high dosage of C.tinctorius were more distinguished.Conclusion： Aqueous extract of Scutellaria and C.tinctorius might have anti-senile action by improving antioxidant capacity of human body.