决明子水提液对高血压小鼠血压血脂及肾脏结构的影响

Effects of Agueous of Cassia obtusifolia on Blood Pressure, Blood Lipid and Renal Structure of Cold-induced Hypertensive Mice

目的:检测决明子水提液对冷激诱导的高血压小鼠血压、血脂及肾脏结构的影响。方法:制备决明子水提液备用。实验小鼠分为正常对照组、冷激对照组、冷激决明子饲喂组(5,10,20 m g.kg-1),对照组相应给予等量生理盐水,连续饲喂18 d。检测各组小鼠血压、血脂含量,切片观察肾脏结构。结果:冷激对照组血压显著高于正常对照组(P<0.01),决明子水提液饲喂组小鼠的收缩压、舒张压、平均动脉压与正常对照组相比,差异不显著。冷激对照组(TG)含量显著高于正常对照组(P<0.01),而冷激饲喂组(TG)含量明显降低(P<0.05);冷激饲喂(10,20 mg.kg-1)组TC含量降低(P<0.05);冷激饲喂(10mg.kg-1)组LDL与HDL含量均降低,其他饲喂组与冷激对照组相比含量虽有降低但无统计学意义。冷激对照组肾小管管壁水肿,内腔极度缩小,肾小球明显胀大,肾小囊腔显著变窄,决明子饲喂组肾小管水肿基本消失,内腔明显增大,肾小球、肾小囊结构趋于正常。结论:决明子水提液具有降血压、降血脂、改善病变肾脏结构的作用。

Objective：To explore the effects of agueous extracts of Cassia obtusifolia on blood pressure,blood lipids,and kidney structure in cold-induced hypertensive mice.Method： C.obtusifolia water extracts was made.Miec were divided into normal control group,cold-stress control group,and cold-stress feeding groups（5,10,20 m g.kg-1.DW）,The control groups were given physiological saline as the control.After 18 days,blood pressure was measured by carotid intubatton and the contents of serum trireactive glyceride（TG）,total cholesterol（CHO）,low density lipoprotein（LDL）,high density lipoprotein（HDL）were also detected.Mice＇s kidney were observed by routine histology inspection.Result： The blood pressure of cold-stress control group increased significantly compared with normal control group（P 〈 0.01）.Cold-stress feeding groups＇ systolic pressure,diastolic pressure and mean arterial pressure were not evident compared with normal control group.The content of TG in cold-stress control group increased than it in normal control group（P 〈 0.01）,and the content of TG,TC in cold-stress feeding groups decreased compared with cold-stress control group（P 〈 0.05）.The content of LDL and HDL in cold-stress feeding group（10 mg.kg-1） decreased compared with cold-stress control group（P 〈 0.05）,and the others had no statistical significance compared with cold-stress control group.Renal histologic changes caused by cold-stress show severely enlarged glomeruli,narrowed Bowman＇s spaces,and renal tubule edema.Nephrons of the cold-stress feeding groups were similar to the control group.Conclusion：The C.obtusifolia water extracts possesses the effects of decreasing blood pressure and modulating blood lipid contents and improvingpathological changes of renal structure.