半枝莲醇提工艺的优化及体外抗氧化活性评价的研究

Studies on Optimum Ethanol Extraction Process and Antioxidant Activities in vitro of Scutellaria barbata

目的:用正交试验法对半枝莲的乙醇提取工艺进行优化研究,通过体外试验对提取物的抗氧化活性进行评价。方法:以浸膏得率、总黄酮和野黄芩苷从生药中的提取率为评价指标,选择乙醇体积分数(%)、乙醇用量(倍)、提取时间(h)和提取次数(次)为考察因素,利用正交试验L9(34)确定半枝莲的最佳醇提工艺。基于氧化还原反应原理的FRAP法用于总抗氧化能力的评估,DPPH法用于评价自由基清除能力。结果:最佳工艺条件为用药材10倍量的75%乙醇加热回流提取3次,每次1 h。半枝莲提取物以浓度依赖关系发挥着很好的抗氧化作用,对DPPH自由基具有较强的清除和抑制作用。结论:该提取工艺具有较佳的重复性和可行性。半枝莲醇提物显示了较好的抗氧化活性,值得进一步开发研究。

Objective：To study the optimum ethanol extraction process and antioxidant activities in vitro of Scutellaria barbata D.Don.Method：The optimum ethanol extraction process was established with the orthogonal design.The extraction rate of extractum,total flavonoids and scutellarin from crude material were chosen as the as-sessment guideline.Antioxidant activities in vitro of the extract were evaluated by FRAP assay and DPPH assay compared with Trolox.Result：The optimum ethanol extraction process conditions were as follows：10 fold of 75% ethanol was added,to be extracted 3 times by reflux and 1 h per time.The extract of S.barbata exerted antioxidant role with dose-dependent relation in the two systems through oxidation-reduction reaction and inhibition of free radi-cal.Conclusion：Validation studies proved that the ethanol extraction process had good reproducibility and feasibil-ity.The ethanol extract of S.barbata exhibited a strong antioxidant activity in vitro,which is worthy of further stud-ies.