正交试验优化“柴胡-白芍”药对提取工艺的研究

Study on Extracting Technology for Radix Bupleuri Matched Paeoniae Alba by Orthogonal Test

目的:优选出柴胡-白芍药对的最佳提取工艺。方法:采用正交设计法,通过高效液相色谱法以柴胡皂苷a、d和芍药苷等苷类成分的含量为指标综合评价,优化提取工艺。结果:最佳提取工艺为加10倍量70%乙醇,提取2次,每次1.5 h。结论:优选的提取工艺稳定,可行。

Objective：To optimize extraction process of the combination of Radix Bupleuri and Paeoniae Alba.Method：The proper extracting condition was optimized by orthogonal tests with the amount of saikosaponin A,D and paeoniflorin as index.Result：The proper extracting condition was that alcohol concentration was 70 per-cent,and extracted with 10 fold alcohol for 2 times,1.5 hour each time.Conclusion：Optimized process condition was scientific,stable and feasible.