芥子不同品种的色谱对应鉴别

Chromatographic Corresponding Identifications in Different Botanical Origins of Sinapis Semen

目的:研究建立芥子不同品种的化学色谱对应鉴别方法。方法:以4-羟基-苄基芥子苷、4-羟基苯甲酰胆碱和芥子碱复盐为对照品,采用TLC和HPLC鉴别。结果:黄芥子、白芥子的TLC和HPLC图谱有明显差异,白芥子能检出3个对照品,黄芥子仅能检出芥子碱复盐,2种色谱方法鉴定结果一致。结论:所建立的TLC和HPLC图谱鉴别方法简便易行,可以快速地鉴别区分黄芥子和白芥子,为芥子不同品种的质量评价方法提供了试验依据。

Objective：To study on the chemical chromatographic corresponding methods in different botani-cal origins of Sinapis semen.Method：The presence of 4-hydroxybenzylglucosinolate,4-hydroxy benzoylcholine and sinapine was identified by TLC and HPLC,respectively.Result：The TLC and HPLC chromatograms showed the obvious differences between the two origins,while three standards were identified in Sinapis alba,and only sinapine was detected in Brassica juncea.Conclusion：The established methods were simple and can be used for evaluating the quality in different botanical origins of Sinapis semen.