反相液相色谱法测定毛头牛蒡不同部位中牛蒡苷和牛蒡苷元的含量

Content Determination of Arctiin and Arctigen from Different Parts of Arctium tomentosum by RP-HPLC

目的:对毛头牛蒡不同部位中牛蒡苷和牛蒡苷元进行含量测定。方法:采用反相液相色谱法,Kromasil C18柱(4.6 mm×250 mm,5μm),流动相甲醇-水(55∶45),检测波长280 nm,流速1.0 mL.min-1。结果:毛头牛蒡根中含有少量牛蒡苷,不含牛蒡苷元;茎、叶柄中几乎不含牛蒡苷及牛蒡苷元;叶、花序中不含牛蒡苷而含有少量的牛蒡苷元;而其种子中二者含量相对较高。结论:该方法简便、准确,可同时测定毛头牛蒡中牛蒡苷和牛蒡苷元的含量,为更好的开发利用毛头牛蒡提供科学依据。

Objective：To establish an HPLC method for determination of different parts of Arctium tomento-sum.Method：Chromatographic separation was achieved by using RP-HPLC system consisting of a Shimadzu LC-6AD and Kromasil C18column（4.6 mm × 250 mm,5 μm）.The mobile phase was consisted of methanol and water（55∶ 45）.Detection wavelength was at 280 nm.The flow rate was set at 1.0 mL.min-1.Result：A small amount of arctiin and no arctigenin was found in the root;while stem and leaf nearly did not contain.Leaves and inflores-cence did not contain arctiin and a few arctigenin.But both ingredients were higher in the fruit than in the leaves and inflorescence.Conclusion：The method was simple and accurate,and it could be used for simultaneous deter-mination of arctiin and arctigenin from Radix Arctium,to provide the basis for a better development of A.tomento-sum Mill.