不同地区市售香附饮片中总黄酮含量测定

Determination of Total Flavonoids in Prepared Medical Herbs of Cyperi Rhizoma from Different Areas

目的:对不同地区香附饮片中总黄酮含量进行测定。方法:采用紫外分光光度法,以芦丁为对照,测定波长507nm。结果:芦丁对照品在3.0～50.0 mg.L-1线性关系良好(r=0.999 8)。10个地区市售香附饮片中总黄酮质量分数一般在0.81%～1.75%,只有上海的最少,为0.42%。结论:香附饮片中总黄酮含量较高。总黄酮含量与香附炮制方法及外观性状有一定相关性。本研究为全面评价香附饮片的质量提供了理论依据。

Objective：To determinate the contents of total flavonoids in the prepared medical herbs of Cy-peri Rhizoma from different areas.Method：The contents of total flavonoids were detected by UV while rutin was used as controller and measurement wavelength was 507 nm.Result：The results showed that rutin had a good Line-ar relationship in the concentrations between 3.0 mg.L-1 to 50.0 mg.L-1（r = 0.999 8）.The contents of total flavonoids in most areas were 0.81%-1.75% except from Shanghai which was 0.42%.Conclusion：The contents of total flavonoids of the prepared medical herbs of Cyperi Rhizoma were higher and it may be used as one of quality index.