二至丸水提物对四氯化碳致小鼠急性肝损伤的保护作用

Protective Effect of Aqueous Extract of Erzhi Pill Against Acute Hepatic Injury Induced by Carbon Tetrachloride in Mice

目的:研究二至丸水提物(aqueous extract of Erzhi Pill,AEEP)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法:将60只昆明种小鼠随机分成6组:正常对照组、四氯化碳损伤模型组、AEEP高、中、低剂量组(19.8,13.2,6.6 g.kg-1)和联苯双酯组(150 mg.kg-1)。AEEP高、中、低剂量组每日ig 1次,共7 d,末次ig后除正常组外,其余所有小鼠予ip 0.1%CCl4 20 mL.kg-1 1次,16 h后处死全部小鼠,收集肝组织及血清标本,测定小鼠血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性;测定肝匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力;计算肝指数并同时对肝组织进行病理学检查。结果:AEEP具有剂量依赖性地降低CCl4致小鼠肝损伤血清ALT,AST升高,降低肝匀浆中MDA的含量,增强SOD的活性;AEEP各剂量组能明显改善肝组织的病理变化。结论:AEEP对四氯化碳造成的小鼠急性肝损伤具有显著的保护作用。

Objective：To study the protective effect of aqueous extract of Erzhi Pill（AEEP） on acute hepatic injury induced by carbon tetrachloride（CCl4） in mice.Method：Sixty Kunming mice were randomly divided into six groups：the normal group,the model group,bifendate group（150 mg.kg-1）,high AEEP group（19.8 g.kg-1）,middle AEEP group（13.2 g.kg-1） and low AEEP group（6.6 g.kg-1）.The treatment groups were orally administered once per day for 7 d separately,whereas the normal and model groups were orally administered with saline.Except normal rats,all the other rats were injected intraperitoneally CCl4 20 mL.kg-1 once.The rats were sacrificed 16 h after CCl4administration.Serum and liver samples were collected for analysis.The acute hepatic injury model was prepared by CCl4 injected intraperitoneally.Then,the therapeutic effects of AEEP on the model were evaluated by the activity determination of serum alanine aminotransferase and asparate aminotransferase（ALT and AST）,superoxide dismutase（SOD） and the content of malondialdehyde（MDA） in liver,and the hepatic pathohistological changes following the treatment.Result：The activities of ALT and AST and the MDA content in liver was significantly increased and the activity of SOD was largely inhibited in the animals ofmodeling group.Following the treatment with AEEP,ALT and AST activities and MDA content were significantly reduced and SOD activity was obviously increased in the mice of treatment group.Furthermore,AEEP could ameliorate the hepatic pathological changes.Conclusion：AEEP have protective effects on acute hepatic injury induced by CCl4in mice.