逍遥散干预抑郁症睡眠障碍模型大鼠海马5-HT_(1A)受体,5-HT_(2A)受体的变化

Influence of Ease Powder Decoction on Expression of Hippocampus 5-HT_(1A) Receptor and 5-HT_(2A) Receptor in Rat Model of Sleep Deprivation Depression

目的:观察逍遥散干预抑郁症睡眠障碍(SDD)大鼠自主行为及海马5羟色胺1A受体(5-HT1A受体),5羟色胺2A受体(5-HT2A受体)mRNA表达变化,探讨其治疗SDD的分子生物学机制。方法:48只Wistar大鼠进行敞箱行为(OFT)试验,根据得分将大鼠随机分为4组:正常组、模型组、米氮平组、逍遥散组。除正常组外,其余各组共接受18 d不同的应激,应激18 d后,进行72 h快眼睡眠剥夺造模(DEM)。每天在应激前1 h按组给药;模型组、米氮平组、逍遥散组分别予等容量生理盐水、米氮平、逍遥散水提液。21 d计算行为得分后处死大鼠,取大鼠海马置液氮中备测。结果:21 d后模型组大鼠自主活动较应激前显著减少(P<0.01)。与模型组比较,逍遥散组及米氮平组大鼠的自主活动明显增加,已达正常组水平;与正常组比较,模型组海马5-HT1AR mRNA表达水平下降,5-HT2AR mRNA表达水平上升(P<0.01);逍遥散组海马5-HT1AR mRNA,5-HT2ARmRNA的表达改善明显,与模型组比较,(P<0.01);而米氮平组与模型组比较无统计学意义,逍遥散组作用明显优于米氮平组(P<0.05)。结论:逍遥散可能通过上调海马5-HT1AR,下调5-HT2AR,调节5-HT再摄取发挥治疗SDD效应。

Objective：To discuss the molecular mechanism of Ease Powder decoction treating on sleep deprivation depression（SDD） by observing the changes of autonomic-behavior and the expression of hippocampus 5-HT1A receptor and 5-HT2A receptor in a rat experimental model of sleep deprivation depression.Method：48 Wistar rats were divided into four groups after first open-field testing（OFT）,they were normal group,model group,mirtazapine group and Ease Powder decoction group.Except the normal group,the other groups were received different stress for 21 days,then made 72 h rapid eye movement（REM）,taken the drug every day before the stress（1 h）.Meanwhile,the model group,mirtazapine group and Ease Powder decoction group were intragastric administration of normal saline,mirtazapine,Ease Powder decoction respectively.At the end of 21 days,all the animals were sacrificed.The hippocampus of rats were taken then kept in liquid nitrogen.Result：Compared with the situation before stress,there is a significant decrease in the autonomic-behavior of SDD after 21 days（P 0.01）.Compared with the model group,the rat＇s autonomic-behavior in Ease Powder decoction group and the mirtazapine group is increased.Compared with the normal group,the expression of 5-HT1AR mRNA in hippocampus of model group is decreased,the expression of 5-HT2A R mRNA is increased,with statistical significance（P 0.05）.Comparison for expression of hippocampus 5-HT1AR mRNA and 5-HT2AR mRNA between the Ease Powder decoction group and the model group is significant（P 0.01）.However,compared with the model group,the mirtazapine group has no statistical significance.Comparison of Ease Powder decoction group and mirtazapine group has statistical significance（P 0.05）.Conclusion：The Ease Powder decoction exert an effect on SDD by regulating the expression of hippocampus 5-HT1AR and 5-HT2AR.