A20基因5′上游序列生物信息学分析

The bioinformation of the 5'-flanking region of A20 gene

目的 A20是一种胞浆瞬时表达蛋白,通过特定的信号通路负反馈调节体内炎症反应。为了解A20基因表达调控炎症的特点,对其5′上游序列进行生物信息学分析,获取基因调控的相关因素。方法从Genebank获取A20基因及其上游序列,利用promter2.0、Transfac 6.0、motif等生物信息学软件分析核转录因子的调控信息。结果结合多种软件分析,A20基因5′上游序列无典型的TATA盒和CAAT盒,且含有多个高度保守的核转录因子结合位点,包括3个与NF-kappa B结合的区域、4个与AP-1结合的区域、1个与HSF结合的位点、1个与Oct-1结合的位点等。结论 A20的表达调控受包括炎症重要核转录因子在内的多种核转录因子的共同调节,且核转录因子之间的协同作用不可忽视。

Objective A20 is the protein of instantaneous expression in the cytoplasm,which takes part in negative adjust inflammation reaction. For understanding the characteristic of A20 in regulating gene expression of inflammation, bioinformaties analyse the A20 gene proximal 5＇-flanking region. Methods The A20 gene and 5＇-flanking region were obtain from Genebank, the transcriptional factor regulation information was analyzed by Using promoter 2.0 and Tansfae6.0 sofwares. Results It do not exist TATA and CAAT box in the 5＇-flanking region of A20 gene. There maybe have three conservative binding sites for NF-κB, four binding sites for AP-1,one binding sites for HSF and one binding sites for Oct-1 . Conclusion many inflammatory transcriptional factors work together for the expression of A20, the synergy among them should not be ignored.