富勒烯赖氨酸衍生物对AHH-1细胞防护效应研究

Study on radio-protection effects of fullerene derivative C_(60)-Lys in AHH-1 cell

本文研究了富勒烯赖氨酸衍生物C60-Lys对AHH-1细胞的辐射防护作用,并对其防护机理进行了初步研究。发现800mg/LC60-lys能提高辐照后AHH-1细胞存活率,在8Gy照射剂量时,给药组与对照组AHH-1细胞存活率分别为64.6%和41.3%。荧光凋亡实验发现,当C60-lys浓度为1200mg/L时,凋亡率为16.3%,这明显低于单纯辐照组的39.2%。在800mg/LC60-lys和4Gy辐照剂量时,给药时间对细胞存活率有显著影响,辐照前给药,AHH-1细胞平均存活率是85.6%,而辐照后给药则是64.1%。C60-lys降低4Gy辐射引起的AHH-1细胞的G2期阻滞及辐射对AHH-1细胞DNA损伤,尤其是减少8-羟基-2'脱氧鸟嘌呤核苷(8-OHdG)含量。

In this paper the radioprotection effects of fullerene derivative （C60-Lys） on AHH-1 cells and its protective mechanism have been investigated.The 800 mg/L C60-lys can improve the survival rate of irradiated AHH-1 cells, which are 64.6% and 41.3% for the irradiation and control groups at 8 Gy dose, respectively.The cell fluorescence images show that the 16.3% apoptosis rate at 1200 mg/L C60-lys is lower than that of the 39.2% irradiation free of C60-lys.In the situation with additive 800 mg/L C60-lys and 4 Gy of irradiation, the dosed time before or after irradiation significantly affects the average survival rates of AHH-1 cells, which are 85.6% and 64.1%, respectively.In the AHH-1 cells treatment with C60-lys can obviously reduce the G2 blockage of AHH-1 cells induced by 4 Gy radiation, DNA damage and amount of 8-OHdG especially.