摘要
以苹果砧木M9为材料,研究了琼脂、激素、NH4+与NO3-离子浓度、架温等对组培苗玻璃化的影响,结果表明:优化配方MS(其中NH4NO30.8g/L,KNO32.28g/L)+BA 0.6mg/L+IBA 0.3mg/L+琼脂8g/L,不仅使M9组培苗的玻璃化程度明显下降,同时还具有很好的分化系数,为M9继代培养的主要配方。
During the plant tissue culture of apple rootstock M9,we did research on the factors,such as agar,hormone,NH4^＋ and NO3-concentration,the temperature of different layer of culture frame,which affected the vitrification of tissue-cultured micro-cuttings.The results are as follows： the modified MS medium（NH4NO3 0.8g/L,KNO3 2.28g/L） ＋ BA 0.6mg/L ＋ IBA 0.3mg/L ＋ agar 8g/L）,could reduce the vitrification rate greatly with satisfactory differentiate rate and was the main subculture medium for M9.
出处
《山东林业科技》
2010年第5期27-30,共4页
Journal of Shandong Forestry Science and Technology
关键词
苹果砧木
组培苗
玻璃化
apple rootstock
tissue-cultured micro-cuttings
vitrification
