微卫星DNA标记用于三疣梭子蟹家系亲子关系的鉴定

Applicability of microsatellite DNA markers in parentage determination of Portunus trituberculatus

以定向交尾技术构建的4个谱系清晰的家系为材料,检测两对微卫星标记Pot17和Pot42在三疣梭子蟹家系鉴别中的应用效果。结果表明,两个位点在4个家系的5个已知父母本和98个后代中共检测出11个等位基因,其中Pot17位点6个,Pot42位点5个。分析显示,上述检出的等位基因频率在0.0291和0.3592之间,Pot17和Pot42位点的期望杂合度分别为0.7740和0.7577,观测杂合度分别为0.6214和0.6796,多态信息含量分别为0.7373和0.7129。根据已知亲本和子代基因型,推断出了4个家系全部亲本的基因型。Pot17和Pot42微卫星标记具有达0.995的较高累积个体识别率,可以用于三疣梭子蟹的亲子关系鉴定。

Based on four families obtained by directional mating techniques,the application of two pairs of microsatellite markers,Pot17 and Pot42,in Portunus trituberculatus parentage determination was explored. Eleven alleles were found at two microsatellite DNA loci （six at locus Pot17 and five at locus Pot42 respectively） by investigating 5 parents and 98 offsprings in the four families. The analysis results showed that the gene frequencies detected by locus Pot17 and Pot42 were between 0.029 1 and 0.359 2,expected heterozygosity was 0.774 0 at locus Pot17 and 0.757 7 at locus Pot42,observed heterozygosity was 0.621 4 at locus Pot17 and 0.679 6 at locus Pot42,and the polymorphism information content （PIC） was 0.737 3 at locus Pot17 and 0.712 9 at locus Pot42,respectively. The results showed that the cumulate power of discrimination （CDP） of Pot17 and Pot42 reached up to 0.995. They are applicable in the Portunus trituberculatus parentage determination.