硒和维生素E对牙鲆生长和非特异性免疫力的影响

Effects of dietary vitamin E and selenium on growth,nonspecific immune responses and disease resistance in Japanese flounder Paralichthys olivaceus

在基础饲料中分别添加维生素E(VE)(60mg/kg标为e或300mg/kg标为E)和硒(Se)(0mg/kg标为s或2.5mg/kg标为S),制成4种试验饲料(s/e,s/E,S/e,S/E)分别饲喂初始体重为38.5±0.15g的牙鲆70d,观察其对生长性能、肝脏及血清中谷胱甘肽过氧化物酶(GSH-Px)活性、非特异免疫力以及抗病力的影响。结果表明,饲喂添加高剂量VE的两组饲料(s/E,S/E),牙鲆的特定生长率和吞噬率明显地提高(P<0.05)。饲喂添加硒的两组饲料(S/e,S/E),牙鲆血液和肝脏的GSH-Px活性显著升高(P<0.05)。70d投喂实验结束后,利用鳗弧菌进行攻毒试验,不添加硒和低剂量VE(s/e)使牙鲆的累积死亡率明显高于其他3组。

A 10-week feeding trial was conducted to investigate the effects of four diets containing different combinations of selenium and vitamin E on the growth performance,nonspecific immune responses of Japanese flounder Paralichthys olivaceus and disease resistance when challenged with Vibrio anguillarum. The fish were fed purified diets with supplemented vitamin E at 60 mg/kg （designated e） or 300 mg/kg （designated E） combination with selenium at 0 mg/kg （designated s） or 3 mg/kg （designated S）. Each diet was fed to triplicate groups of flounder with initial body weight of 38.5±0.15 g. After the final weighing,8 fish were randomly taken from each aquarium and injected with V.anguillarum. Mortality was recorded daily for 7 days and nonspecific immune parameters were monitored in the remaining fish. At the end of the experiment,weight gain and phagocytic activity in blood was significantly greater in those fish fed the s/E and S/E diets compared with those fed the s/e and S/E diets. Glutathione peroxidase activity in serum and liver were significantly higher in fish fed the S diets compared with those fed the s diets. High cumulative mortality was found in fish fed the s/e diet than other dietary groups. These results indicate that no synergistic effect of excessive dietary vitamin E and selenium on growth performance,nonspecific immune responses and disease resistance to V.anguillarum in Japanese flounder.