不同载体固定化海洋微生物酯酶ETM-b的性能比较

Study on immobilization of marine microbial esterase ETM-b with different material

分别采用海藻酸钠包埋法、明胶包埋交联法、壳聚糖吸附交联法制备固定化海洋芽孢杆菌酯酶ETM-b,并对其固定化条件进行了研究。结果发现,壳聚糖制备的固定化酶效果最好,壳聚糖2%、戊二醛浓度1%、小球与酶液4∶3(g/ml)时制备的固定化酶的活性回收最高,达到66%。壳聚糖制备的固定化酶使用10次,相对活性保留70%,具有良好的操作稳定性。固定化酶在非水介质中具有转化α-乙酸萘酯(α-Naphthyl acetate)的能力,在异辛烷、正辛烷、正己烷中活性表现最高。

Esterase from Bacillus sp. was immobilized with sodium alginate,gelatin and chitosan. The enzyme,immobilized with 2% chitosan,1% glutaraldehyde and chitosan microspheres：enzyme loading=4：3 （g/ml） exhibited the highest activity recovery （66%）. The immobilized esterase with chitosan could be reused for 10 times,and 70% of enzyme activity remained after the 10th batch,which exhibited good operational stability. The immobilized esterase can catalyze α-naphthyl acetate in non-aqueous system,and it displayed high activity in isooctane,n-octane and n-hexane.