瑞香狼毒根对长爪沙鼠的急性毒力测试

Acute Toxicity Analyses of Stellera chamaejasme Root in Mongolian Gerbils(Meriones unguiculatus)

本文采用含17%瑞香狼毒(Stellera chamaejasme)根粉末的毒饵,依据孙氏改良寇氏综合计算法,实验室内测定了其对长爪沙鼠(Meriones unguiculatus)的急性毒力。根据预试验结果,采用最低剂量为7.0 g.kg-1,最适剂量梯度比值K=0.65的5个剂量梯度的瑞香狼毒根毒饵饲喂长爪沙鼠。结果显示:试验组试鼠体重明显下降(P=0.029<0.05),完全致死组肝脏重/体重比对照组明显减小(P=0.014<0.05),但肾脏略增大(P=0.102>0.05)。其对长爪沙鼠的LD50为(20.37±0.086)g.kg-1,95%平均可信限为(20.37±7.84)g.kg-1,属弱毒水平。

Both whole Stellera chamaejasme and its extract are demonstrated to have powerful toxicity to some pest insects.The poison bait containing 17 % roots of Stellera chamaejasme was used to test the acute toxicity of Stellera chamaejasme in Mongolian gerbils according to Sun′s improved Kou′s Integrated Way.Mongolian gerbils were treated with 5 treatments of toxicant bait according to pretested results： the lowest dose was 7.0 g/kg and the optimal gradient dose radio was 0.65.Results indicate that the body weight of treatment group was significantly decreased as compared to control（P=0.029〈0.05）.The rate of liver to body weight was significantly decreased in groupⅤ（P=0.014〈0.05,whereas,the percent of kidney to body weight was slightly increased in groupⅤ（P=0.102〉0.05） compared with controls.The LD50 of Stellera chamaejasme in Mongolian gerbils is 20.37±0.086 g/kg.The confidence limit on 95% is（20.37±7.84） g/kg.The acute virulence is slight as relates to the graded standard of toxicant against mice.