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拟南芥抗灰霉病相关基因的克隆与原核表达

Cloning and prokaryotic expression of resistant-related gene of Arabidopsis against Botrytis cinerea
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摘要 为了从拟南芥中获得抗灰霉病相关基因以及进行基因分析,本试验利用RACE技术获得了该基因的cD-NA全长(1190 bp),并用生物信息学方法和Southern Blotting技术明确了该基因是编码372个氨基酸的转录调节/结合蛋白的AT5G67480基因,以单拷贝形式存在于拟南芥基因组中。编码的蛋白含有BTB/POZ结构域,体外诱导表达的蛋白对灰霉病菌的生长有一定的抑制作用。 Full-length cDNA sequence of resistant-related gene of Arabidopsis against Botrytis cinerea was obtained by running RACE.Result of homology indicated that gene identified as AT5G67480 gene,which encoded binding protein or transcription regulator,containing 372 amino acids.AT5G67480 gene is a single copy in Arabidopsis genomic DNA by Southern Blotting analysis.The protein encodes by AT5G67480 gene contains BTB/POZ domain of TAZ zinc protein family.In most cases,protein containing BTB/POZ domain is related to resistance to pathogens,and such protein is resistant to B.cinerea by expression of AT5G67480 gene in E.coli and in vitro inhibition on B.cinerea.
作者 陈展 郝丛丛 李莉 邢继红 董金皋
机构地区 河北农业大学真菌毒素实验室
出处 《河北农业大学学报》 CAS CSCD 北大核心 2010年第5期29-33,共5页 Journal of Hebei Agricultural University
基金 河北省自然科学基金项目(08B021) 河北农业大学9816科技重点项目资助
关键词 拟南芥 灰葡萄孢 RACE 原核表达 Arabidopsis Botrytis cinerea RACE prokaryotic expression
分类号 S432.1 [农业科学—植物病理学]
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参考文献14

  • 1瓮巧云,邢继红,董金皋.拟南芥抗病基因克隆的策略及利用[J].西北农林科技大学学报（自然科学版）,2005,33(B08):220-224. 被引量：3
  • 2Borhan M H,Holub E B,Beynon J L,et al.The Arabidopsis TIR-NB-LRR gene RAC1 confers resistance to Albugo candida (white rust) and is dependent on EDS1 but not PAD4[J].Molecular Plant Microbe Interactions,2004,17(7):711-719.
  • 3Xiao S,Ellwood S,Calis O,et al.Broad-spectrum mildew resistance in Arabidopsis thaliana mediated by RPW8[J].Science,2001,291(5501):118-120.
  • 4Mengiste T,Chen X,Salmeron J,et al.The Botrytis susceptible1 gene encodes an R2R3MYB transcription factor protein that is required for biotic and abiotic stress responses in Arabidopsis[J].Plant Cell,2003,15(11):2551-2565.
  • 5Veronese P,Chen X,Bluhm B,et al.The BOS loci of Arabidopsis are required for resistance to Botrytis cinerea infection[J].Plant Journal,2004,40(4):558-574.
  • 6Nandi A,Moeder W,Kachroo P,et al.Arabidopsis ssi2-conferred susceptibility to Botrytis cinerea is dependent on EDS5 and PAD4[J].Molecular Plant Microbe Interactions,2005,18(4):363-370.
  • 7Veronese P,Nakagami H,Bluhm B,et al.The membrane-anchored botrytis-induced kinase1 plays distinct roles in Arabidopsis resistance to necrotrophic and biotrophic pathogens[J].Plant Cell,2006,18(1):257-273.
  • 8邢继红,瓮巧云,董金皋.拟南芥抗灰霉病菌相关基因的差异表达分析[J].植物病理学报,2006,36(6):562-565. 被引量：5
  • 9Marchler-Bauer A,Anderson J B,Deweese-Scott C,et al.CDD:a curated Entrez database of conserved domain alignments[J].Nucleic Acids Res,2003,31(1):383-387.
  • 10Bardwell V J,Treisman R.The POZ domain:a conserved protein-protein interaction motif[J].Genes Dev,1994,8(14):1664-1677.

二级参考文献49

  • 1Bryan G T, Wu K S, Farrall L, et al. A single amino acid differences distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta[J].The Plant Cell, 2000, 12(1): 2033-2045.
  • 2Wang Z X, Yano M, Yamanouchi U, et al. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes[J].Plant J., 1999, 19(1):55-64.
  • 3He Z H, Shen Z T. Near-isogenic pairs of Indica rice with blast ( B1 ) resistance genes[ J ]. Intern. Rice Res. Newsl. , 1990, 15(1): 7.
  • 4Sambrook E F, Fritsch T, Maniatis T. Molecular cloning: a laboratory manual (2nd ed. ) [M]. New York:Cold Spring Harbor Laboratory Press, 1989.
  • 5Cheong Y H, Kim C Y, Chun H J. Molecular cloning of a soybean class Ⅲ beta-1, 3-glucanase gene that is regulated both developmentally and in response to pathogen infection [ J ]. Plant Science ( Shannon ),2000, 154(1): 71-81.
  • 6Molina A, Mena M, Carbonero P, et al. Differential expression of pathogen-responsive genes encoding two types of glycine-rich proteins in barley [ J ]. Plant Mol.Biol., 1997, 33(5) : 803 -810.
  • 7Godoy A V, Lazzaro A S, Claudia A C, et al. Expression of a Solanum tuberosum cyclophilin gene is regulated by fungal infection and abiotic stress conditions [J]. Plant Science, 2000, 152(1) : 123 -134.
  • 8Matilde J E, Pere P. Plant cell wall glycoproteins and their genes [ J ]. Plant Physiol. Biochem. , 2000, 38( 1/2 ) : 97 - 108.
  • 9Deswal R, Sopory S K. Glyoxalase I from Brassica juncea is a calmodulin stimulated protein [ J ]. Biochimica et Biophysica Acta (BBA)/Molecular Cell Research, 1999, 1450(3) : 460-467.
  • 10Rairdan G J, Donofrio N M, Delaney T P. Salicylic acid and NIM1/NPR1-independent gene induction by incompatible Peronospora parasitica in Arabidopsis[J]. MPMI, 2001, 14(10) : 1235-1246.

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河北农业大学学报
2010年 第5期

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