HPLC测定天佛参口服液中蟾酥的含量

Content Determination of Venenum Bufonis in Tianfushen Oral Solution by HPLC

目的：建立HPLC同时测定天佛参口服液中华蟾酥毒基和脂蟾毒配基的含量。方法：采用Waters SymmetryShield^TM RP18色谱柱（4．6mm×250mm，5μm）；以乙N-0．5％磷酸二氢钾（38：62）为流动相；检测波长为296nm；流速为1．0mL·min^-1结果：在建立的色谱条件下，华蟾酥毒基的回归方程为Y=1000000 X-648．2，r=0．9990，表明华蟾酥毒基在0．0139～0．695μg与峰面积积分值呈良好线性关系；脂蟾毒配基的回归方程为Y：5000000X＋42394，r=0．9995，脂蟾毒配基在0．0210～1．0525μg与峰面积积分值呈良好线性关系。结论：本法简便、快捷，准确度高，专属性强，重现性好，可用于华蟾酥毒基、脂蟾毒配基的含量测定。

Objective： To establish a HPLC method to determine the contents of cinobufagin and resibufogenin for chief components of venenum bufonis in tianfushen oral solution. Methods： The method were determined by HPLC with Waters SymmetryShieldTM RP18 （4. 6 mm× 250 mm,5μm） as column, a mixture of acetonitrile ：0. 5 % potassium dihdyrogen phosphate （38：62） as mobile phase, UV detector at 296nm and flow rate of 1.0mL.min^-1 Results： The calibration curves are linear in the range of 0. 013 9 -0. 695 μg for cinobufagin（r =0. 999 0）, 0. 021 0 - 1. 052 5 μg （r = 0. 999 5） for resibufogenin, respectively, regression equations were Y = 1 000 000 X - 648. 2 and Y = 5 000 000 X ＋ 42 394, respectively. Conclusion： The method is simple, rapid and accurate, which can be used to determine chief components of venenum bufonis in tianfushen oral solution.