参龙健脑胶囊对脑缺血损伤大鼠学习记忆能力及皮层反应性氮中介物的影响

Effect of Shenlongjiannao Capsule on Learning and Memory and Reactive Nitrogen Intermediates in Cerebral Cortex of Cerebral Ischaemia Rats

目的探讨参龙健脑胶囊对脑缺血大鼠学习记忆能力以及大脑皮层反应性氮中介物的影响。方法利用永久性结扎大鼠双侧颈总动脉的方法制备脑缺血模型,50只大鼠分为假手术组、模型组、脑复康阳性对照组、参龙健脑胶囊大、小剂量组。4周后分别进行Morris水迷宫实验及测定大脑皮层一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)含量。结果参龙健脑胶囊能明显提高脑缺血大鼠的学习记忆能力,降低NO、iNOS含量。结论参龙健脑胶囊能降低脑缺血大鼠大脑皮层NO、iNOS含量,对学习记忆能力有明显提高作用。

Objective To explore the effects of Shenlongjiannao capsule on the learning and memory of the cerebral ischaemia model rat as well as reactive nitrogen intermediates in the cerebral cortex.Methods The cerebral ischaemia animal model was established by two-vessel occlusion. 50 rats were randomly divided into 5 groups：control group with sham operation,model group,positive control group with pisacetam,Shenlongjiannao capsule groups （low and high dosage）. After four weeks,the learning and memory function was tested with Morris water maze,and the contents of nitric oxide（NO） and inducible nitric-oxide synthsae（iNOS） in cerebral cortex were measured.Results After treatment,the learning and memory ability improved significently and the contents of NO and iNOS in cerebral cortex decreased in Shenlongjiannao capsule groups.Conclusion Shenlonjiannao capsule can improve the learning and memory of rats with cerebral ischaemia by lowering the activity of NO and iNOS in the cerebral cortex.