莫诺苷对局灶性脑缺血再灌注大鼠皮层IL-1β的影响

Effects of Morroniside on IL-1β in Rat Cortex with Focal Cerebral Ischemia/Reperfusion

目的研究山茱萸有效成分莫诺苷对局灶性脑缺血再灌注大鼠皮层IL-1β的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型,缺血30min,再灌注72h。以秋水仙碱为阳性对照药,采用酶联免疫吸附法(ELISA)检测大鼠脑皮层炎症因子IL-1β的变化。结果莫诺苷可剂量依赖性地明显降低由脑缺血再灌引起的IL-1β含量增加(P<0.001)。结论莫诺苷能通过增强大鼠皮层抗炎能力发挥神经保护作用。

Objective To investigate the effects of morroniside on IL-1β in rat cortex with focal cerebral ischemia/reperfusion.Methods The animal model was induced by occlusion of middle cerebral artery with suture embolus,ischemia for 30 min,and reperfusion for 72 h in rats. The content of IL-1β was detected with enzyme linked immunosorbent assay（ELISA）.Results Compared with sham group,the content of IL-1β increased obviously in model rat. Compared with model group,morroniside（30 mg/kg,90 mg/kg,270 mg/kg） and colchicine （0.06 mg/kg） decreased the content of IL-1β significantly （P〈0.001）.Conclusion Morroniside may protect the cortex from inflammatory factor IL-1β.