隔药饼灸对兔动脉粥样硬化斑块中基质金属蛋白酶-2,9 mRNA表达的影响

Moxibustion with Medicine Cake on mRNA Expression of Matrix Metalloproteinases-2,9 in Artherosclerosis Plaques of Rabbits

目的观察隔药饼灸对兔动脉粥样硬化(AS)中基质金属蛋白酶-2,9(MMP-2,9)表达的影响,探讨隔药饼灸对兔动脉粥样硬化斑块稳定性的影响。方法将75只新西兰大耳白兔,通过高脂饲料喂养及免疫损伤法建立兔AS模型。然后随机分为5组,每组15只,即:空白组(空白对照组);模型组(AS模型组);直接灸组(AS模型+艾炷直接灸);隔药饼灸组(AS模型+隔药饼灸);西药组(AS模型+阿托伐他汀)。运用原位杂交法检测兔动脉粥样硬化斑块中MMP-2,9mRNA的表达。结果隔药饼灸、直接灸和西药均能抑制兔动脉粥样硬化斑块中MMP-2和MMP-9mRNA的表达(P<0.01或P<0.05);隔药饼灸组和西药组MMP-2和MMP-9mRNA的表达低于直接灸组(P<0.01或P<0.05)。结论隔药饼灸、直接灸和西药通过抑制兔动脉粥样硬化斑块中MMPs的表达,起到稳定动脉粥样硬化斑块的作用,且隔药饼灸和西药抑制MMPs表达的作用优于直接灸。

Objective To observe the effect of moxibustion with medicine cake on mRNA expression of matrix metalloproteinases-2,9 （MMP-2,MMP-9）in artherosclerosis （AS） plaques of rabbits,and to explore the effect of moxibustion with medicine cake to the stability of artherosclerosis plaques of rabbits.Methods AS rabbit models were established by feeding high-fat diet. 75 New Zealand big-eared rabbits were divided into 5 groups,blank group （blank control group）,model group （AS model group）,direct moxibustion group （AS model ＋ moxa cone direct moxibustion）,moxibustion on medicine cake group （AS model ＋ moxibustion on medicine cake）,western medicine group （AS model ＋ atorvastatin）,15 rabbits in each group. Then the mRNA expression of MMP-2,MMP-9 was detected in the atherosclerotic plaques of rabbits with in situ hybridization.Results The expressions of MMP-2,MMP-9 in the atherosclerotic plaques of rabbits were all be controlled in direct moxibustion group,moxibustion on medicine cake group,western medicine group （P〈0.01 or P〈0.05）. The expressions of MMP-2 and MMP-9 in the moxibustion on medicine cake group and western medicine group were obviously lower than those of the direct moxibustion group.Conclusion The expression of MMP-2,MMP-9 in the atherosclerotic plaques of rabbits can all be controlled in direct moxibustion group,moxibustion on medicine cake group,and western medicine group; as well as the plaques can all be made stable. The efficacy in the moxibustion on medicine cake group and western medicine group is superior to that of the direct moxibustion group.