细胞问黏附分子基因多态性与老年原发性高血压相关性研究

Intercellular adhesion molecule genes polymorphisms and essential hypertension in elder people

目的研究细胞间黏附分子（ICAM）系统的3个基因（ICAM1、ICAM2和ICAM3基因）的7个单核苷酸多态性（SNPs）位点与原发性高血压的相关性。方法采用TaqMan荧光定量PCR技术，对中国上海地区313例老年高血压（EH）患者和252例老年血压正常（NT）者进行基因分型，单位点及单倍型分析。结果发现ICAM2基冈的A／G多态分布与高血压相关，G等位基因频率在EH组高于NT组（0．35vs0．23，P〈0．001）；G等位基因携带者（AG＋GG基因型）左室重量指数高于AA基因型（108．60±26．36）g，m2VS（100．30±23．32）g／m2，（P=0．023）。末发现其他SNP分布与高血压相关。结论ICAM2基因A／G多态与老年原发性高血压相关。

Objective This study was to investigate whether the single nucleotide polymorphisms （SNPs） in three genes of ICAM （ICAM1, ICAM2 and ICAM3 genes） are associated with essential hypertension （EH） through a lager case-co.ntrol study. Methods We performed the genotyping analyses, haplotype analyses and genotype with phenotypic analyses in 313 elderly hypertensive patients and 252 healthy elderly people. Seven SNPs in 3 genes of ICAM system were detected using the TaqMan fluorescent quantitation PCR methods. Results SNPs analyzing and association study showed that the A/G allele distributions of ICAM2 gene were different between the groups. The frequency of G allele was significantly higher m the EH group compared with the NT group （0.35 vs 0.23, P〈0.001）. The left ventricular mass index was higher in G carriers than in AA genotype （108.60±26.36） g/m2 vs （100.30±23.32） g/m2, （P=0.023）. For the other SNPs, no significantly difference of distribution of alleles, genotypes or haplotypes was observed between EH and NT groups. Conclusion ICAM2 gene A/G polymorphism might be associated with hypertension in Chinese.