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卡介苗多糖核酸对哮喘大鼠淋巴液和血液CD4^+CD25^+Foxp3^+调节性T细胞的影响 被引量：5

The effect of BCG-PSN on CD4^+CD25^+Foxp3^+ Treg in blood and lymph of asthma rats
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摘要 目的:探讨卡介苗多糖核酸(BCG-PSN)对哮喘大鼠淋巴液和血液调节性T细胞数量及功能的影响。方法:将SD大鼠随机分为对照组、哮喘组和BCG-PSN组,分别收集不同时间点大鼠淋巴液和血液,采用流式细胞仪(FCM)检测CD4+CD25+Foxp3+调节性T细胞(CD4+CD25+Foxp3+Treg)百分率,酶联免疫吸附试验(ELISA)检测淋巴液和血浆白介素10(IL-10)和转录生长因子β1(TGF-β1)浓度。结果:各组在各时间点其淋巴液中CD4+CD25+Foxp3+Treg百分率、IL-10水平均较血液明显升高。哮喘组大鼠淋巴液和血液中CD4+CD25+Foxp3+Treg百分率、IL-10、TGF-β1浓度均较对照组显著降低(P<0.05)。BCG-PSN组淋巴液和血液中CD4+CD25+Foxp3+Treg百分率和IL-10水平较哮喘组明显升高(P<0.05),与对照组比较无显著性差异;而TGF-β水平在48小时较对照组和哮喘组明显升高(P<0.05)。结论:哮喘大鼠淋巴液和血液存在明显CD4+CD25+Foxp3+Treg数量及功能不足。BCG-PSN可能通过增加哮喘大鼠外周血和淋巴液中CD4+CD25+Foxp3+Treg的数量及其产生IL-10和TGF-β水平,增强免疫抑制效应,从而发挥抑制哮喘炎症的作用。 Objective： To discuss the effect of BCG-PSN on CD4^＋CD25^＋Foxp3^＋ Treg in blood and lymph at different time points. Methods： SD rats were iandomly provided into control group, asthma group and BCG-PSN group. The blood and lymph were collected at different time points. Percentage of CD4^＋CD25^＋Foxp3^＋ Treg was measured by flow cytometry（FCM）. The levels of IL-10 and TGF-β were determined by ELISA. Results：The percentage of CD4^＋CD25^＋Foxp3^＋ Treg and the level of IL-10 in lymph were higher than in blood at different time points. In asthma group, the percentage of CD4^＋CD25^＋Foxp3^＋ Treg and the levels of IL- 10 and TGF-β in Lymph and blood were significantly lower than in control group（ P 〈 0. 05 ）. In BCG-PSN group, the percentage of CD4^＋CD25^＋Foxp3^＋ Treg and the level of IL-10 in lymph and blood were significantly higher than asthma group（ P 〈 0.05）, but there was no significant difference with those of control group, and the level of TGF-β was significantly higher than in asthma group and control group at 48 h. Conclusion：The number and the function of the CD4^＋CD25^＋Foxp3^＋Treg are significantly insufficient in lymph and blood of asthma rats. BCG-PSN may enhance the immune suppressive effects by increasing the number of C!）4 ＋ CD25 ＋ Foxp3 ＋ Treg in the peripheral blood and lymph of asthmatic rats and the IL- 10 and TGF-β levels seerected by CD4^＋CD25^＋Foxp3^＋Treg, and thus play a role in inhibiting inflammation in asthma.
作者 石涛 冯学斌 赵志旭 张瑾锦
机构地区 滨州医学院儿科学教研室 滨州医学院实验医学研究中心
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2010年第10期932-936,共5页 Chinese Journal of Immunology
基金 山东省教育厅科技计划项目(No.J07YE14)
关键词 哮喘 卡介苗多糖核酸 CD4+CD25+Foxp3+Treg IL-10 TGF-Β Asthma BCG-PSN CD4^＋CD25^＋Foxp3^＋Treg IL- 10 TGF-β
分类号 R562.2 [医药卫生—呼吸系统]
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参考文献20

  • 1迟磊,符州,戴继宏,王莉佳.过敏性哮喘大鼠模型的建立[J].重庆医学,2003,32(4):429-431. 被引量：60
  • 2Lewkowich I P,Herman N S,Schleifer K W et al.CD4+CD25+T cells protect against experimentally induced asthma and alter pulmonary dendritic cell phenotype and function[J].J Exp Med,2005;202(11):1549-1561.
  • 3Wang L H,Lin Y H,Yang J et al.Insufficient Increment of CD4+CD25+ regulatory T cells after stimulation in vitro with allergen in allergic asthma[J].Int Arch Allergy Immunol,2009;148(3):199-210.
  • 4Hartl D,Koller B,Mehlhorn A T et al.Quantitative and functional impairment of pulmonary CD4+CD25hi regulatory T cells in pediatric asthma[J].Allergy Clin Immunol,2007;119(5):1258-1266.
  • 5Lee J H,Yu H H,Wang L C et al.The levels of CD4+CD25+ regulatory T cells in paediatric patients with allergic rhinitis and bronchial asthma[J].Clin Exp Immunol,2007;148(1):53-63.
  • 6李营营,冯学斌,吴福玲,李洪波,邢朝品.支气管哮喘大鼠淋巴液Th1与Th2类细胞因子变化及其与支气管肺泡灌洗液、血浆水平比较[J].现代免疫学,2009,29(5):413-417. 被引量：3
  • 7邢朝品,冯学斌,宋晓冬,张丽霞,刘文波.支气管哮喘大鼠淋巴液中淋巴细胞线粒体跨膜电位变化及地塞米松对其干预的影响[J].滨州医学院学报,2009,32(4):249-252. 被引量：4
  • 8Kronenberg M,Rudensky A.Regulation of immunity by self-reactive T cells[J].Nature,2005;435(7042):598-604.
  • 9Birebent B,Lorho R,Lechartier H et al.Suppressive properties of human CD4+ CD25+ regulatory T cells are dependent on CTLA-4 expression[J].Eur J Immunol,2004;34(12):3485-3496.
  • 10Presser K,Schwinge D,Wegmann M et al.Coexpression of TGF-β1 and IL-10 enables regulatory T cells to completely suppress airway hyperreactivity[J].J Immunol,2008;181(1):7751-7758.

二级参考文献24

  • 1刘艳凯.淋巴回流在机体稳态调节中的意义[J].中国微循环,2006,10(4):307-310. 被引量：12
  • 2Bisset LR , Schmid-Grendelmeier P. Chemokines and their receptors in the pathogenesis of allergic asthma : proga'ess and perspective [ J]. Curr Opin Pulm Med, 2005,11 ( 1 ) :35-42.
  • 3Woodside KJ, Hu M, Meng T,et al. Differential effects of Interleukin- 2 blockade on apoptosis in naive and activated human lymphocytes [ J ]. Transplantation,2003, 75 (10) : 1631-1635.
  • 4Verburg J, Hollenbeck PJ. Mitochondrial membrane potential in axons increasesWith local nerve growth factor orsemaphor insigaling [ J]. Neurosci ,2008,28 (33) :8306-8315.
  • 5Pak HN , Qayyum M , Kim DT, et al. Mesenchymal stem cell injection induces cardiac nerve sprouting and increased tenascin expres sion in a Swine model of myocardial infarction [ J ]. Cardiovasc Elec trophysiol, 2003, 14 (8) :841-848.
  • 6L undy SK, Berlin AA, M artens TF, et al. Deficiency of regulatry B ceils increases allergic airway inflammation [ J ]. Inflamm Res, 2005, 54 (12) : 514-521.
  • 7Tong JK, Hozefa S, Bandulwala, et al. Fas-positive T cells regulate the resolution of airway inflammation in a murine model of asthma [J]. J Exp Med,2006, 203(5):1173-1184.
  • 8Ray A, Cohn L. Altering the Thl/Th2 balance as a therapeutic strategy in asthmatic diseases [J]. Curr Opin Investig Drugs, 2000, 1: 442-448.
  • 9Mempel TR, Henrickson SE, von Andrian UH. T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases[J]. Nature, 2004, 427: 154-159.
  • 10Pala P, Message SD, Johnston SL, et al. Increased aeroallergen-specific interleukin-4-producting T cells in asthmatic adults[J]. Clin Exp Allergy, 2002, 32: 1739-1744.

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中国免疫学杂志
2010年 第10期

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