恩施地区乙型肝炎患者HBV DNA YMDD变异的检测

Detection of HBV DNA YMDD Mutation in Patients with Chronic Hepatitis B in Enshi Autonomous Prefecture

目的了解恩施地区慢性乙型肝炎（乙肝）患者HBV DNA YMDD变异的情况。方法检测该地区100例乙肝患者ALT水平、HBV DNA载量、HBV YMDD变异,对其HBV DNA YMDD变异情况进行分析。结果 100例乙肝患者中检出HBV DNA YMDD变异共15例（阳性率15%）,已服用拉米夫定者60例,检出YMDD变异12例（阳性率20%）,未服用拉米夫定患者40例,检出YMDD变异3例（阳性率7.5%）。15例YMDD变异患者HBV DNA在5.21×10^4-1.34×10^8copies/ml,ALT水平在38-1100U/L。结论恩施地区乙肝患者YMDD变异可在拉米夫定治疗过程中出现,也可天然存在;尚未发现恩施地区乙肝患者性别、年龄、HBV DNA载量。

Objective To investigate the existence of HBV DNA YMDD mutation in patients with chronic hepatitis B in Enshi Autonomous prefecture.Methods The ALT level,HBV DNA,and HBV DNA YMDD mutation in the patients with chronic hepatitis B were detected and analyzed.Results HBV DNA YMDD mutation was found in 15 out of 100 patients with hepatitis B（positive rate 15%）.HBV DNA YMDD mutation was found in 12 out of 60 patients receiving lamivuding therapy（positive rate 20%）,but HBV DNA YMDD mutation was found in 3 out of 40 patients without lamivuding therapy（positive rate 7.5%）.In the 15 patients with HBV DNA YMDD mutation,HBV DNA and ALT level were 5.21×10^4～1.34×10^8 copies/ml and 38～1100 U/ L respectively.Conclusion The existence of HBV DNA YMDD mutation in patients with chronic hepatitis B in Enshi Autonomous Prefecture may occur during the process of lamivuding therapy or may exist naturally.The existence of HBV DNA YMDD in patients with chronic hepatitis B mutation in Enshi Autonomous Prefecture is unrelated with gender,age,serum viral load or ALT level.