竹灵消嫩茎再生系统建立研究

Research on Establishing the Regeneration System of Cynanchum Paniculatum

以竹灵消嫩茎为材料,进行了愈伤组织诱导和分化,试管苗的生根、移栽和移植的研究,建立起竹灵消嫩茎的再生体系。结果证明:MS+BA0.5mg.L-1+24,-D 1.8mg.L-1-2.0mg.L-1是愈伤组织诱导的理想培养基;MS+AgNO30.6mg.L-1+KT0.7mg.L-1+NAA0.1 mg.L-1是愈伤组织分化培养的理想培养基;将不定芽下部切口在50mg.L-1IAA无菌溶液中处理10min后接种到1/2MS0培养中进行生根培养的方法是生根培养的理想方法;移植到山坡上的试管苗长势旺盛,根系发达,当年开花结实。

The induction and differentiation of the callus were studied for young stem of Cynanchum inamoenum and then rooting and transplanting of tube plantlets were also studied,in the end an asexual system of Cynanchum inamoenum were successfully carried out in the experiment.The results showed that MS＋BA0.5mg.L^-1＋2,4-D 1.8mg.L^-1-2.0mg.L^-1 was the optimum medium for callus induction.The ideal medium for callus differentiation was MS＋AgNO30.6mg.L^-1＋KT0.7mg.L^-1＋NAA0.1mg.L^-1.The method of rooting was that transfer into the 1/2MS media after the cut of adventitious bud was treated in the 50mg.L^-1IAA for 10min.The seedling grew well with flourishing root and blooming and seeding in the former years.