TGF-β1 rs1800469基因多态性与新疆汉族原发性高血压的相关性研究

Relationship between the rs1800469 Polymorphisms of TGF-β1 Gene and Essential Hypertension in the Han of Xinjiang

为研究转化生长因子β1(TGF-β1)基因单核苷酸多态(SNP)rs1800469及血浆水平与新疆汉族原发性高血压(EH)的关系。采用整群抽取随机抽样的方式,以新疆沙湾县732名汉族人(EH组365人,对照组367人)为研究对象,进行流行病学调查和临床检查,并采集血样。用双抗体夹心法(ELISA试剂盒)测量TGF-β1血浆浓度。SNaPshot方法进行基因分型。rs1800469基因型及等位基因在EH组和对照组分布差异无显著性(P>0.05)。TGF-β1血浆水平在EH组与对照组各基因型和等位基因之间无差异(P>0.05)。结果表明:在新疆汉族人群中TGF-β1基因rs1800469位点存在C/T分子变异,与原发性高血压缺乏关联。同时TGF-β1基因rs1800469位点多态性与TGF-β1血浆水平不相关。

We investigate the relationship between the single nucleotide polymorphism rs1800469 in transforming growth factor-β1（TGF-β1） gene,protein levels and essential hypertension（EH） in the Han population in Xinjiang.Based on the case-control study,we selected 732（365 EH patients,367 controls） Han Chinese people from the Boertonggu countryside of Shawan region in the Xinjiang Uygur Autonomous Region of China by random cluster sampling.After questionnaires and physical examinations,we collected blood samples,and the blood levels of TGF-β1 were quantified using sandwich ELISA.The polymorphisms of TGF-β1 in the studygroups were detected with SNaPshot system.The frequencies of genotypes and alleles of rs1800469 had no differences between EH and controls（P0.05）.There were no differences of TGF-β1 levels between the different genotypes and alleles in rs1800469 of TGF-β1 gene（P0.05）.The result showed there was a C to T substitution at rs1800469 of TGF-β1 gene,but there was a lack of association between the mutation of TGF-β1 gene and essential hypertension in the Han of Xinjiang.There was no association between the rs1800469 and TGF-β1 blood levels in them.