氯丹和灭蚁灵污染场地土壤对蚯蚓的毒性效应研究

Toxicity Effects of Chlordane and Mirex Contaminated Soil on Earthworm(Eisenia foetida)

以赤子爱胜蚓(Eisenia fetida)为供试生物,氯丹和灭蚁灵污染场地土壤为供试土壤,以蚯蚓体重及体内蛋白质含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性为指标,进行了不同暴露时间(1、3、7、14 d)下场地土壤中氯丹和灭蚁灵污染胁迫对蚯蚓的毒性效应研究。结果表明,随着暴露时间的延长,蚯蚓体重在氯丹和灭蚁灵作用下受到显著抑制,蚯蚓体内蛋白质、SOD和CAT活性对氯丹和灭蚁灵响应不同,其敏感性大小为CAT>SOD>蛋白质;在一定暴露时间内,根据暴露-剂量效应关系,表明氯丹浓度为14.13 mg.kg-1、灭蚁灵浓度为4.14 mg.kg-1可能是使蚯蚓CAT活性达到最大值的临界浓度,同时也是SOD活性受到抑制的临界浓度,超过该临界浓度可能对蚯蚓产生生态毒性效应,这为场地风险评价和修复提供了基础数据。

An ecotoxicity experiment had been performed by using earthworm（Eisenia fetida）as the test organism.Effects of a chlordane and mirex contaminated soil on body weight,activities of superoxide dismutase（SOD）,Catalase（CAT）and protein of earthworm（Eisenia foetida） were investigated.Results showed that body weight of the earthworm had been significantly inhibited by chlordane and mirex with increase in exposure time.Levels of protein in vivo,SOD and CAT activity in response to chlordane and mirex showed different sensitivities as the follow-ing order：CATSODprotein.According to a dose-response relationship in this study,chlordane at 14.13 mg.kg-1 soil and mirex at 4.14 mg.kg-1 soil might be critical concentrations which led to the highest activity of earthworm CAT as well as obvious inhibition of SOD activity.Eco-toxicity effects on the earthworm might occur when the critical concentrations of chlordane and mirex in the soil were exceeded.The results of this study would provide helpful data and information for risk assessment and remediation of the contaminated site soil.