高糖诱导骨髓来源MAPC细胞TGF-β1表达并干扰其分化趋势的研究

Regulation of Differentiation of MAPCs via High Glucose-induced Expression of TGF-β1

利用骨髓来源MAPC细胞(Multipotent Adult Progenitor Cell)作为细胞模型,通过检测高糖对骨髓来源MAPC细胞TGF-β1表达水平的影响,探讨高糖干预MAPC细胞TGF-β1表达的机制及其对MAPC细胞分化趋势的影响.研究发现,高糖可以上调特异性平滑肌标记物α-SMA和SM22-α表达水平,同时抑制内皮细胞特异性标记物vWF、CD31以及Flk1表达水平,从而诱导MAPC细胞向平滑肌细胞方向分化,并且高糖是通过调控MAPC细胞TGF-β1表达及分泌来影响其自身分化趋势的,其机制是高糖通过ERK1/2信号途径抑制STAT3(Tyr705)磷酸化,最终干预MAPC细胞TGF-β1表达水平.

Multipotent adult progenitor cells（MAPCs）derived from bone marrow stem cell was a kind of cell model.Different methods were used to study expression of TGF-β1 in MAPCs under high glucose.It was to release the mechanism about regulation of differentiation of MAPCs by high glucose inducing expression of TGF-β1.Results indicated that expression of the special markers of smooth muscle cells（α-SMA and SM22α）were up-regulated under high glucose,but expression of endothelial markers（vWF,CD31 and Flk1）were suppressed by high glucose.It meant that high glucose induced differentiation of MAPCs into smooth muscle cells.And the mechanism was that expression of TGF-β1 was increased through inhibition of STAT3 （Tyr705）by enhanced ERK1/2 signaling in MAPCs under high glucose.